PaperDifferential regulation of monocyte/macrophage cytokine production by pressure
Section snippets
Cells and cell cultures
The human monocytic cell line THP-1 was obtained from the American Type Culture Collection (Rockville, MD). THP-1 cells were maintained in RPMI-1640 (Gibco, Grand Island, NY), supplemented with 10% fetal bovine serum (Sigma, St. Louis, MO), L-glutamine (200 mmol/L), and 2-mercapto-ethanol (5 × 10−5 mol/L) (Sigma). MonoMac-1 cells were maintained in RPMI-1640 supplemented with 10% fetal bovine serum, L-glutamine (200 mmol/L), nonessential amino acids, and 1 mmol/L sodium pyruvate.
As previously
Pressure effect on cytokine production by THP-1 cells
We previously reported that exposure to constant low (20 mm Hg) extracellular pressure enhanced serum-opsonized latex bead uptake by PMA-differentiated THP-1 macrophages and primary isolated human peripheral monocytes [5], [6]. We now examined the effect of extracellular pressure on TNF-α and IL-1β release from the human THP-1 monocytic cell line. PMA-differentiated (macrophage-like) or unstimulated (monocyte-like) THP-1 cells were cultured under ambient pressure (consistent with extracellular
Comments
Normal interstitial tissue pressure in human beings is 20 to 30 mm Hg and generally decreases in acute infection and inflammation [1], [2], [3], [4]. Although infection or edema in closed compartments may increase tissue pressure by 5 to 80 mm Hg [16], [17], [18], interstitial extracellular pressure generally decreases in infected or inflamed tissue lacking such special conditions. Edema formation in unconstrained inflamed or infected tissues actually may be associated with decreases in
Acknowledgment
Supported in part by National Institutes of Health grant RO1 DK60771 (M.D.B.).
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