Original articleGeneral thoracicExpression of Bone Morphogenetic Proteins in Human Lung Carcinomas
Section snippets
Reagents
Primary antibodies mouse anti-BMP-2 (clone 65529), mouse anti-BMP-4 (clone 66119), mouse anti-BMP-6 (74219), mouse anti-BMP-7, and goat anti-growth differentiation factor 5 (anti-GDF-5) were purchased from R & D Systems (Minneapolis, MN). Recombinant proteins BMP-2, BMP-4, BMP-6, and GDF-5 were purchased from R & D Systems and were used as positive controls on Western blot analysis.
Tissue Specimens
Tissues were obtained directly from the operating room and immediately frozen in liquid nitrogen. All samples had
Expression of BMP Family Members in NSCLC
We found, by Western Blot analysis, high expression of the mature BMP-2 protein in the majority of NSCLC with little or no detectable expression in normal lung tissue (Fig 1). The BMP-2 was found to be expressed in all but one of the NSCLC examined (Table 1). The mature BMP-6 protein was detected in all the NSCLC examined, however it was expressed only slightly higher than that of normal lung tissue (Fig 1 and Table 1). Only low levels of expression of BMP-4 and GDF-5 were seen in a small
Comment
This paper demonstrates a high and frequent overexpression of BMP-2 in lung carcinomas. The mature active form of BMP-2 was aberrantly expressed in 98% of the NSCLC examined. BMP-2 is expressed in all types of lung carcinomas including both epithelial and neuroendocrine derived tumors. All of the adenocarcinomas and squamous cell carcinomas were found to overexpress BMP-2. The same level of BMP-2 expression was seen in both adenocarcinomas and squamous cell carcinomas. In this small analysis of
Acknowledgment
This study was funded by NIH Grant No. CA91919-01A1 and The Thoracic Surgery Foundation.
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