Biochimica et Biophysica Acta (BBA) - General Subjects
ReviewNanoscale engineering of extracellular matrix-mimetic bioadhesive surfaces and implants for tissue engineering☆
Section snippets
Tissue engineering and biomimetic strategies overview
The main goal of tissue engineering and regenerative medicine strategies is to restore the function of damaged tissues by delivering a combination of cells, biological factors and a biomaterial scaffold on which these cells must adhere, organize and develop similarly to native tissue (Fig. 1). In vivo, cell fates are determined by a complex interaction of nanoscale physical and chemical signals. Therefore, scaffolds for tissue engineering often incorporate biosignals to create a controlled,
Cellular interactions with extracellular environment
Signals from the extracellular microenvironment that may be incorporated into biomaterials (Fig. 2) fall into three major categories: (1) insoluble extracellular matrix (ECM) macromolecules, (2) diffusible/soluble molecules, and (3) cell–cell receptors.
From micro to nano in biomaterials
Over the past few decades, techniques for creating nanoscale features, patterns and particles have emerged. Although these techniques were initially applied to electronics fabrication, they have more recently been used to pattern and immobilize proteins and peptides with nanoscale precision for applications such as tissue engineering, drug delivery and biosensing. Like nanotechnology, the interdisciplinary field of tissue engineering is also fledgling, and began approximately two decades ago
Techniques to nanopattern peptides and proteins
Nanoscale control of ECM-derived peptides and proteins have primarily been used for non-regenerative medicine applications, including biosensing, drug delivery and for model systems to study cell functions such as adhesion and spreading. However, given that integrins and focal adhesions exist on submicron size scales, there is a compelling rationale for using nanotechnology in bioadhesive tissue engineering applications. The following strategies have been used to control and pattern proteins
Nanoscale engineering of proteins and peptides for bioadhesion
How can nanoscale engineering of ECM-derived adhesive peptides be used to enhance cellular response for tissue regeneration? Although this field is admittedly in its infancy, some significant works have already been completed which demonstrate the promise of this approach. Nanoscale bioadhesive tissue engineering strategies which have been employed can be classified under three major categories:
- 1)
Nanoscale control of adhesive and modulatory peptide domains to retain full bioactivity of parental
Conclusion and future outlook
The fast-evolving fields of tissue engineering and nanotechnology have begun to converge, giving rise to new methods of directing cell fates by controlling the presentation of ECM-derived peptides with nanoscale precision. In recent decades, a range of exciting new strategies for peptide nanopatterning have been developed with important benefits and capabilities such as patterning more complex, higher resolution, multi-peptide patterns with greater spatial control, as well as rapid and low-cost
References (134)
- et al.
- et al.
Profiling genes expressed in human fetal cartilage using 13, 155 expressed sequence tags
Osteoarthr. Cartil.
(2003) Integrins: bidirectional, allosteric signaling machines
Cell
(2002)- et al.
Focal adhesions: structure and dynamics
Biol. Cell
(2000) Integrins: versatility, modulation, and signaling in cell adhesion
Cell
(1992)- et al.
Structure of human platelet membrane glycoproteins IIb and IIIa as determined by electron microscopy
J. Biol. Chem.
(1985) - et al.
Structure of the integrin alpha2beta1-binding collagen peptide
J. Mol. Biol.
(2004) - et al.
Identification in collagen type I of an integrin alpha2 beta1-binding site containing an essential GER sequence
J. Biol. Chem.
(1998) - et al.
2.0 Å crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region
Cell
(1996) - et al.
The short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function
J. Biol. Chem.
(1994)
The minimal essential sequence for a major cell type-specific adhesion site (CS1) within the alternatively spliced type III connecting segment domain of fibronectin is leucine-aspartic acid-valine
J. Biol. Chem.
A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth
J. Biol. Chem.
Identification of a second active site in laminin for promotion of cell adhesion and migration and inhibition of in vivo melanoma lung colonization
Arch. Biochem. Biophys.
Extracellular matrix as a biological scaffold material: structure and function
Acta Biomater.
Delivering growth factors for therapeutics
Trends Pharmacol. Sci.
Using self-assembled monolayers to model the extracellular matrix
Acta Biomater.
Self-assembled oligopeptide nanostructures for co-delivery of drug and gene with synergistic therapeutic effect
Biomaterials
Hybrid bone implants: self-assembly of peptide amphiphile nanofibers within porous titanium
Biomaterials
Engineering RGD nanopatterned hydrogels to control preosteoblast behavior: a combined computational and experimental approach
Biomaterials
Multi-scale modeling to predict ligand presentation within RGD nanopatterned hydrogels
Biomaterials
Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands
Biophys. J.
Microcontact printing of novel co-polymers in combination with proteins for cell-biological applications
Biomaterials
Three-dimensional micropatterning of bioactive hydrogels via two-photon laser scanning photolithography for guided 3D cell migration
Biomaterials
Micromolding of shape-controlled, harvestable cell-laden hydrogels
Biomaterials
Molecular Biology of the Cell
Cell adhesion differentially regulates the nucleocytoplasmic distribution of active MAP kinases
J. Cell Sci.
Integrin signaling
Science
Fibronectin matrix regulates activation of RHO and CDC42 GTPases and cell cycle progression
J. Cell Biol.
Geometric control of cell life and death
Science
Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton
Annu. Rev. Cell Biol.
Electron microscopy and structural model of human fibronectin receptor
EMBO J.
Identification of an alternatively spliced site in human plasma fibronectin that mediates cell type-specific adhesion
J. Cell Biol.
A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor
Biochemistry
Creating biomimetic micro-environments with synthetic polymer–peptide hybrid molecules
J. Biomater. Sci., Polym. Ed.
Polymeric growth factor delivery strategies for tissue engineering
Pharm. Res.
Nanoparticulate systems for growth factor delivery
Pharm. Res.
Mimicking cell–cell interactions at the biomaterial-cell interface for control of stem cell differentiation
J. Biomed. Mater. Res. A
Synthetic biomimetic hydrogels incorporated with ephrin-A1 for therapeutic angiogenesis
Biomacromolecules
Tissue engineering
Science
Electrospun nanofiber scaffolds: engineering soft tissues
Biomed. Mater.
Engineering substrate topography at the micro- and nanoscale to control cell function
Angew. Chem. Int. Ed. Engl.
Nanobiomaterial applications in orthopedics
J. Orthop. Res.
Nanoscopic Pd line arrays using nanocontact printed dendrimers
Langmuir
Biological bottom-up assembly of antibody nanotubes on patterned antigen arrays
J. Am. Chem. Soc.
Dip-pen nanolithography on (bio)reactive monolayer and block-copolymer platforms: deposition of lines of single macromolecules
Small
Micelles by self-assembling peptide–conjugate amphiphile: synthesis and structural characterization
J. Pept. Sci.
Sequence-specific binding of DNA to liposomes containing di-alkyl peptide nucleic acid (PNA) amphiphiles
Langmuir
Self-assembled peptide amphiphile nanofibers conjugated to MRI contrast agents
Nano Lett.
Self-assembling peptide amphiphile-based nanofiber gel for bioresponsive cisplatin delivery
Mol. Pharmacol.
Cited by (111)
A novel strategy for fabrication of antibacterial Kirschner wire via Langmuir-Blodgett assembly
2023, Surface and Coatings TechnologyA nano-structured TiO<inf>2</inf>/CuO/Cu<inf>2</inf>O coating on Ti-Cu alloy with dual function of antibacterial ability and osteogenic activity
2022, Journal of Materials Science and TechnologyThe fate of stem cells within smart biomaterials and constructs
2022, New Trends in Smart Nanostructured Biomaterials in Health SciencesFuture of nanotechnology in tissue engineering
2022, Tissue Engineering: Current Status and ChallengesSurface functionalization of biomaterials for cell biology applications
2020, Biomaterials for Organ and Tissue Regeneration: New Technologies and Future Prospects
- ☆
This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine.