Adiponectin differentially regulates cytokines in porcine macrophages☆
Section snippets
Materials and methods
Porcine blood-derived macrophage isolation and culture. Blood collected from barrows at slaughter (666 mL) into Alsever’s solution (334 mL) was centrifuged in 50 mL conical tubes at 900g for 20 min at 20 °C. The buffy coats from five conicals of blood were mixed in a new conical with 30 mL Hanks’ balanced salt solution (HBSS) at pH 7.4 (Gibco-BRL, Grand Island, NY). Twenty microliters of the buffy coat plus HBSS mixture was layered atop 15 mL of Ficoll–Paque (Sigma Chemicals, St. Louis, MO) in a
Adiponectin regulates TNFα and IL6 in porcine blood-derived macrophages
The regulation of TNFα and IL6 release by macrophages pretreated with adiponectin for 24 h prior to exposure to LPS for an additional 24 h is presented in Figs. 1A and B, respectively. Adiponectin at 10 μg/mL was sufficient to suppress cytokine release by approximately 50% (P<0.05). Similar results were obtained for IL6 in that 10 μg/mL adiponectin abrogated (P<0.05) the LPS response in macrophages. These findings confirm the findings of Yokota et al. [2] and extend the anti-inflammatory activity
Conclusions
In conclusion, we have confirmed that adiponectin attenuates the LPS-induced expression and release of TNFα from macrophages, and show for the first time that this hormone also down-regulates IL6 while up-regulating IL10. Furthermore, we provided solid evidence that the anti-inflammatory activity of adiponectin is achieved in part by inhibition of NFκB activation and suppression of ERK1/2 activity. Collectively, these data substantiate the potential therapeutic benefit of adiponectin in the
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Purdue Agriculture Research Program No. 17342.