Biochemical and Biophysical Research Communications
Lecithinized brain-derived neurotrophic factor promotes the differentiation of embryonic stem cells in vitro and in vivo
Section snippets
Materials and methods
Animals. All animals were housed at a constant temperature (23 ± 1 °C) and humidity (50–60%) with free access to a standard diet and water. The animal room had a 12-h light/dark cycle. All of the experimental procedures were in accordance with the St. Marianna University guidelines for the welfare of animals.
Synthesis of PC-BDNF. BDNF was kindly provided by Sumitomo Pharmaceutical (Osaka, Japan). PC-BDNF was synthesized according to the method of Igarashi et al. [13]. Analysis by matrix-assisted
Results
First, the affinity of the retinoic acid-treated neural precursor cells for BDNF and PC-BDNF was examined (Table 1). The incubation of 3 × 105 cells with 1 ng/ml 125I-BDNF resulted in the binding of 0.007 ± 0.001 ng ligand. The same dose of 125I-PC-BDNF resulted in the binding of 0.028 ± 0.001 ng ligand, which is 4-fold higher than for BDNF. Similar results were obtained regardless of the amount of 125I-BDNF added. At 100 ng/ml of 125I-BDNF, there was a total binding of 0.164 ± 0.022 ng of ligand, of which
Discussion
The present study demonstrated that PC-BDNF not only had a much higher affinity for neural precursor cells but was also a more potent inducer for neural differentiation. Of particular importance is that transplanted PC-BDNF-treated cells were more effective than BDNF-treated cells at promoting functional motor recovery following SCI.
The fact that PC-BDNF had improved affinity for the neural cells is not surprising because we have already shown that derivatization of other proteins, such as
Acknowledgments
This study was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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