Antiviral activity of ovotransferrin derived peptides☆
Section snippets
Materials and methods
Proteins. Bovine milk lactoferrin was from Armor Proteins (Bretagne, France); human lactoferrin was from Sigma Chemical (St. Louis, MO); and hOtrf was purified by chicken white egg, as previously described [28]. In all cases, protein purity was checked by silver-stained SDS–PAGE. Protein concentration was determined by UV spectroscopy, assuming an extinction coefficient (280 nm, 1% solution) of 1.51 for lactoferrins [29] and 1.10 for ovotransferrin [28]. Before biological assays, all proteins
Results and discussion
Three bLf-derived peptides possess antiviral activity: lactoferricin B (bLf17–41) [22], [23], [24], fragments ADRDQYELL (bLf222–230) and EDLIWK (bLf264–269) [27], though their antiviral activity was much lower than that of the intact protein. The hOtrf fragments displaying sequence and/or structural homologies with antiviral bLf fragments are listed in Table 1. No fragment was identified in hOtrf having sequence homology with bLf fragment lactoferricin B (bLf17–41). On the contrary, two
Acknowledgments
The work was partially supported by Ministero Istruzione Universitàe Ricerca, Project COFIN 2002. Prof. P. Valenti (II University of Naples, Italy) and Prof. L. Seganti (University of Rome “La Sapienza”) are gratefully acknowledged for helpful discussions.
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Abbreviations: bLf, bovine lactoferrin; hOtrf, hen ovotransferrin; MDV, Marek’s disease virus.