Biochemical and Biophysical Research Communications
A lipid raft-associated 67 kDa laminin receptor mediates suppressive effect of epigallocatechin-3-O-gallate on FcεRI expression☆
Section snippets
Materials and methods
Reagents. EGCG was purchased from Kurita Water Industries (Tokyo, Japan). Mouse anti-human FcεRI α chain antibody CRA-1 was obtained from Kyokuto seiyaku (Tokyo, Japan). Mouse IgG2b, used as negative control, was bought from Dako Cytomaition (A/S, Denmark). Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG antibody was purchased from Protos Immunoresearch (Burlingame, CA). Mouse anti-phosphorylated ERK1/2 antibody and rabbit anti-ERK1/2 antibody were obtained from Santa Cruz
67LR is extensively associated with the plasma membrane microdomain lipid rafts
Because the 67LR expression in human basophils has not yet been reported, we first examined the expression of 67LR on the cell surface of the human basophilic KU812 cells. Flow cytometric analysis using the 67LR antibody MLuC5 showed that KU812 cells expressed the 67LR on the cell surface (Fig. 1A). To further examine the association of 67LR with lipid rafts, a study of raft integrity was performed using MβCD, a cholesterol-removing agent that disturbs raft structure. After treatment with MβCD,
Discussion
The aim of the present study was to broaden the understanding of EGCG signaling, and a role of the 67LR in the EGCG-induced regulation of FcεRI expression and in the MAPK signaling pathway in human basophilic KU812 cells was examined. Among the many laminin-binding proteins, the 67LR is a high affinity non-integrin laminin receptor [21]. The interaction of cancer cells with laminin has been implicated in tumor metastasis and invasiveness, and the 67LR is thought to be involved in this process
Acknowledgments
This work was supported in part by Grants from Research and Development Program for New Bio-industry Initiatives (to H.T.). We are grateful to Dr. Takeshi Shimomura for helpful discussion. The authors thank Perry Seto for proofreading the manuscript.
References (48)
- et al.
Abolition of anaphylaxis by targeted disruption of the high affinity immunoglobulin E receptor alpha chain gene
Cell
(1993) - et al.
Lipid raft-associated catechin suppresses the FcϵRI expression by inhibiting phosphorylation of the extracellular signal-regulated kinase1/2
FEBS Lett.
(2004) - et al.
Localization of platelet-derived growth factor-stimulated phosphorylation cascade to caveolae
J. Biol. Chem.
(1996) - et al.
Type IV collagen-binding proteins of neutrophils: possible involvement of L-selectin in the neutrophil binding to type IV collagen
Blood
(1996) - et al.
Anti-sense RNA of 32-kDa laminin-binding protein inhibits attachment and invasion of a human colon carcinoma cell line
J. Surg. Res.
(1992) - et al.
Expression of the 37-kDa laminin binding protein in murine lung tumor cell correlates with tumor angiogenesis
Cancer Lett.
(2000) - et al.
Detection of blood group antigens utilizing immobilized antibodies and surface plasmon resonance
J. Immunol. Methods
(1997) - et al.
Detection of porphyrin using a short peptide immobilized on a surface plasmon resonance sensor chip
Biosens. Bioelectron.
(2001) - et al.
Neurological mechanisms of green tea polyphenols in Alzheimer’s and Parkinson’s diseases
J. Nutr. Biochem.
(2004) - et al.
Integrins and GTPases in tumour cell growth, motility and invasion
Trends Cell. Biol.
(1998)
Integrin-mediated tyrosine phosphorylation and redistribution of paxillin during neuronal adhesion
Exp. Cell Res.
Cholesterol depletion of caveolae causes hyperactivation of extracellular signal-related kinase (ERK)
J. Biol. Chem.
Covalently immobilized laminin peptide Tyr-Ile-Gly-Ser-Arg (YIGSR) supports cell spreading and co-localization of the 67-kilodalton laminin receptor with alpha-actinin and vinculin
J. Biol. Chem.
Epigallocatechin-3-O-gallate disrupts stress fibers and the contractile ring by reducing myosin regulatory light chain phosphorylation mediated through the target molecule 67 kDa laminin receptor
Biochem. Biophys. Res. Commun.
Complete structure and expression in transfected cells of high affinity IgE receptor
Nature
A tea catechin suppresses the expression of the high-affinity IgE receptor FcεRI in human basophilic KU812 cells
J. Agric. Food Chem.
Antiallergic tea catechin, (−)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses FcεRI expression in human basophilic KU812 cells
J. Agric. Food Chem.
Functional rafts in cell membranes
Nature
A detergent-free method for purifying caveolae membrane from tissue culture cells
Proc. Natl. Acad. Sci. USA
Evidence for budding of human immunodeficiency virus type 1 selectively from glycolipid-enriched membrane lipid rafts
J. Virol.
A receptor for green tea polyphenol EGCG
Nat. Struct. Mol. Biol.
Gene for an extracellular matrix receptor protein from Pneumocystis carinii
Proc. Natl. Acad. Sci. USA
The 67 kDa laminin receptor as a prognostic factor in human cancer
Breast Cancer Res. Treat.
Overexpression of the 67-kD laminin receptor correlates with tumour progression in human colorectal carcinoma
J. Pathol.
Cited by (0)
- ☆
Abbreviations: EGCG, (−)-epigallocatechin-3-O-gallate; 67LR, 67 kDa laminin receptor; siRNA, small interfering RNA; RNAi, RNA interference; ERK1/2, extracellular signal-regulated kinase 1/2; FcεRI, high-affinity IgE receptor; MAPK, mitogen-activated protein kinase; SPR, surface plasmon resonance; MβCD, methyl-β-cyclodextrin.