Isolation and identification of mesenchymal stem cells from human lipoma tissue

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Abstract

Lipoma is a benign neoplasm of normal fat cells that appears as a soft, movable swelling, often with a slight yellowish coloration. Human mesenchymal stem cells (MSCs) that have been isolated from bone marrow, blood, and other adult tissues including adipose tissue have the potential to be useful candidates for therapy. No literature had reported about stem cells from lipoma tissue. Here, a new cell culture method is described and utilized to greatly accelerate the growth rate and prolong the lifespan of lipoma-derived MSCs. Cells produced in early cultures display characteristics similar to those previously reported for multipotential stem cells, including a high frequency of anchorage-independent growth in soft agar and a lack of gap junctional intercellular communication in cell types with serpiginous morphology. These cells can differentiate into adipocytes, osteoblasts, and chondrocytes after induction. In conclusion, lipoma-derived stem cells possessing the characteristics of MSCs are described for the first time.

Section snippets

Materials and methods

Cell culture media. The unique feature of our cell culture method is the use of a low calcium medium and agents known to affect cellular redox states, as mentioned below. The mesenchymal stem cells were developed from human lipoma tissue using a modified MCDB 153 medium (Keratinocyte-SFM, Gibco-Invitrogen Corporation) supplemented with 2 mM N-acetyl-l-cysteine (NAC; Sigma A8199) and 0.2 mM l-ascorbic acid 2-phosphate (Asc 2P; Sigma A8960). This medium was referred to as K-NAC medium in our

Development of cell culture from lipoma tissue

After one day, the culture of minced lipoma tissue in the modified Eagle’s medium (D-medium, as described in materials and methods) with 10% FBS showed most of the cells or cell aggregates remaining in suspension, including many erythrocytes. After washing to remove these cells, few attached single cells or cell clumps were observed. After a change to K-NAC medium with 5% FBS, these attached cells proliferated actively and became near confluence after one week in 25-cm2 flasks. Morphologically,

Acknowledgment

We are deeply thankful to the help of Miss Samantha Benton in the preparation of this manuscript.

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    These authors contributed equally to this work.

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