Biochemical and Biophysical Research Communications
Expression of vascular cell adhesion molecule-1 indicates the differentiation potential of human bone marrow stromal cells
Section snippets
Cells and tissue samples
ihBMSCs were established by sequential transduction of the hTERT and Bmi1 genes [13], and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma–Aldrich, St. Louis, MI) with 10% fetal bovine serum (FBS, Hyclone, South Logan, UT). Subcloning of ihBMSCs was performed by limited dilution, and 100 clones were established and cultured under the same conditions.
Primary human BMSCs (designated as huBM) were isolated from the bone marrow taken from iliac crests of donors, who received orthopedic
Heterogeneous differentiation potential of ihBMSC clones
One hundred single-cell derived clones were established from the parental ihBMSC by limited dilution. No significant difference was observed among clones in terms of growth potential (data not shown). The adipogenic, osteogenic, and chondrogenic potential of each clone was determined by the standard induction method and categorized as either positive or negative based on the definition described in Materials and methods section. Five clones showed tri-directional differentiation, and 78 clones
Discussion
CD106/VCAM-1 is a cell surface glycoprotein, which binds to the α4β1 and α4β7 integrins [19]. A deficiency of CD106/VCAM-1 results in embryonic death with the absence of chorion-allantois fusion or multiple abnormalities in the heart in mice, but no significant abnormality was found in mesenchymal tissues during the embryonic stage [20]. CD106/VCAM-1 is associated with homing of HSCs, and CD106/VCAM-1-positive BMSCs, which may be MSCs, keep HSCs in their niche [21], [22]. The binding to
Acknowledgments
The authors thank Drs. Koichi Nishijo, and Tatsuya Ishibe for advice, and Drs. Takeshi Sakamoto and Hiromu Ito for clinical samples. This work was supported by the New Energy and Industrial Technology Development Organization (NEDO) with a project entitled Development of Evaluation Technology for Early Introduction of Regenerative Medicine, and also by Grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science, from the Ministry of Education, Culture, Sports,
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