Sp1 and CREB regulate basal transcription of the human SNF2L gene
Section snippets
Materials and methods
Cell culture. The rat pheochromocytoma PC12 cells were cultured on collagen coated dishes in RPMI 1640 medium supplemented with 15% horse serum, 2.5% fetal calf serum and antibiotics. HeLa cells and C6 cells were maintained in Dulbecco’s modified Eagle’s medium, supplemented with 10% fetal bovine serum and antibiotics. Cells were incubated in the humidified incubator equilibrated with 5% CO2 at 37 °C and passaged using standard cell culture techniques.
Plasmids and constructs. A 855 bp fragment
Identification of the putative SNF2L promoter region
To isolate the human SNF2L promoter we performed a BLAST search of human genome sequence using the human SNF2L cDNA (GenBank Accession No. NM_003069) as a query. The 5′ sequence of the SNF2L cDNA perfectly matched to a genomic sequence (GenBank Accession No. NW_927721). Computer-based analysis of this region with MatInspector software failed to find any canonical TATA box or CAAT-like sequence. However, several potential transcription factor binding motifs were identified in the promoter region
Acknowledgments
This work was supported by National Basic Research Program of China (973 Program) Grant 2007CB512001 and National Science Fund Grant 30671163.
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