Biochemical and Biophysical Research Communications
Bone marrow stroma cells are susceptible to prion infection
Section snippets
Materials and methods
Isolation and culture of MSCs. Adult male Wistar rats, 8 weeks old, were killed and the femurs and tibias were dissected out. Isolation of the bone marrow was performed according to the method described by Azizi et al. [7]. The ends of the bones were cut and the marrow was extruded with 5 ml of alpha-MEM (Sigma, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS), 100 μg/ml kanamycin, or 100 U/ml penicillin and 100 μg/ml streptomycin. The bone marrow cells were incubated at 37 °C with 95%
Results and discussion
MSCs have unique characteristics. They grow well ex vivo without transformation and are multipotential progenitor cells which can be used for auto-cell transplant therapy8. We isolated and subcultured bone marrow cells, and were able to obtain relatively uniform cell types from human, rat and bovine samples, but not from those of mouse and hamster. (Fig. 1A). The rat bone marrow cells grew for more than 2 years (100 passages), but the human cells stopped dividing at about 15 passages, and the
Acknowledgments
We thank Dr. Yasushi Miyazaki, Dr. Hisako Furukawa, and Dr. Susumu Shirabe for support during the CJD patients’ bone marrow biopsies. We thank Dr. T. Yokoyama for providing mouse-BSE agent. We also thank Dr. Norbert Zilka and Dr. Kazuto Shigematsu for helping the experiments. This study was partially supported by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (06-4) and a Grant from the Ministry of Health, Labor and
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