Elsevier

Biomaterials

Volume 31, Issue 1, January 2010, Pages 99-105
Biomaterials

Oxidative stress in the brain of mice caused by translocated nanoparticulate TiO2 delivered to the abdominal cavity

https://doi.org/10.1016/j.biomaterials.2009.09.028Get rights and content

Abstract

In order to study the mechanisms underlying the effects of TiO2 nanoparticles on the brain, ICR mice were injected with nanoparticulate anatase TiO2 (5 nm) of various doses into the abdominal cavity daily for 14 days. We then examined the coefficient of the brain, the brain pathological changes and oxidative stress-mediated responses, and the accumulation of nanoparticulate anatase TiO2 and levels of neurochemicals in the brain. The results showed that high-dose nanoparticulate anatase TiO2 could induce some neurons to turn into filamentous shapes and others into inflammatory cells. The concentration of nanoparticulate anatase TiO2 in the brain was increased as increases in nanoparticulate anatase TiO2 dosages used. The oxidative stress and injury of the brain occurred as nanoparticulate anatase TiO2 appeared to trigger a cascade of reactions such as lipid peroxidation, the decreases of the total anti-oxidation capacity and activities of antioxidative enzymes, the excessive release of nitric oxide, the reduction of glutamic acid, and the downregulated level of acetylcholinesterase activities. We concluded that TiO2 nanoparticles injected at the abdominal cavity could be translocated into the brain and in turn caused the brain injury.

Introduction

In recent years, TiO2 nanoparticles have increasingly been used in the fields of paints, waste water treatment, sterilization, cosmetics, food additive, bio-medical ceramic and implanted biomaterials largely due to its appropriate physicochemical properties. However, it is these unique characteristics such as small sizes, large surface per mass and high reactivity that nanoparticulate anatase TiO2 can enter the human body quickly, and then imposes potential risks on human health [1], [2]. Several studies have reported that inhaled or injected nanosize particles enter systemic circulation and migrate to various organs and tissues, and exert adverse effects [2], [3], [4], [5], [6], [7], [8]. The administration of TiO2 nanoparticles at 5 g/kg body weight (BW) to mice increased liver weight, and produced hepatocyte necrosis [8]. We recently showed that nanoparticulate anatase TiO2 could cause liver injury and oxidative stress of mice after daily abdominal cavity injection for 14 consecutive days [9], [10], [11]. Nanoparticles can cross the blood–brain barrier [12] and enter (in low numbers) the central nervous system (CNS) of the exposed animals [5], [7]. For instance, largemouth bass that lived in the water containing 500 ppb fullerene were shown to have induced severe brain damages [3]. After the 60-day dermal exposure to hairless mice, TiO2 nanoparticles could penetrate the skin, and was detectable in the brain, although no induced pathological changes were observed [13]. Moreover, TiO2 nanoparticles were shown to stimulate ROS generation in the brain microglia and cause neuron damages in vitro [14]. However, the oxidative toxicity of TiO2 nanoparticles in the brain has not been well studied in vivo to date.

The brain is highly vulnerable to oxidative stress due to its high metabolic rate, the reduced capacity for cellular regeneration (low levels of endogenous scavengers, e.g., vitamin C, catalase, superoxide dismutase), and numerous cellular oxidative stress targets (i.e., lipids, nucleic acids, and proteins) [15]. For instance, nano-sized Fe2O3 particles significantly elevated glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and constitutive NO synthase (cNOS), but decreased the ratio of reduced glutathione (GSH) versus oxidized glutathione (GSSG), thereby causing neurodendron degeneration, membranous structure disruption and lysosome increase in the mouse olfactory bulb [16]. The mice displayed a slight brain lesion after administration of TiO2 nanoparticles (25, 80 and 155 nm) by oral gavage, for the vacuoles as well as the fatty degeneration were observed in the hippocampus of the brain [8]. Wang et al. found that the intranasally instilled TiO2 nanoparticles could migrate into the CNS, deposited in the hippocampus region, and caused oxidative stress and inflammation responses [17]. To gain new insights into the mechanisms underlying the oxidative brain damages caused by nanoparticulate anatase TiO2, here we investigate the accumulation of nanoparticulate anatase TiO2 and the pathological changes in the brain following injection to mice, assess the antioxidant response, and examine the effects of neurochemicals including acetylcholinesterase (AChE) activity, and levels of glutamic acid (Glu) and nitric oxide (NO) in the mouse brain.

Section snippets

Chemicals and preparation

Nanoparticulate anatase TiO2 was prepared via controlled hydrolysis of titanium tetrabutoxide as described previously [18]. In a typical experiment, 1 mL of Ti(OC4H9)4 dissolved in 20 mL of anhydrous isopropanol was added dropwise to 50 mL of double-distilled water adjusted to pH 1.5 with nitric acid under vigorous stirring at room temperature. Then, the temperature was raised to 60 °C and kept 6 h for better crystallization of TiO2 nanoparticles. The resulting translucent colloidal suspension was

Coefficient of brain to body weight

Table 1 shows the coefficients of the brain to body weight, which were expressed as milligrams (wet weight of tissues)/grams (body weight). No significant differences were found in the body weight of seven groups. With increasing dosages, the coefficients of the brain to body weight were decreased gradually, and those of 50, 100, and 150 mg/kg BW nanoparticulate anatase TiO2-treated groups and 150 mg/kg BW bulk TiO2-treated group were significantly lower than that of the control (p < 0.05 or p < 

Discussion

While mice that were treated with nanoparticulate anatase TiO2 and bulk- TiO2 did not significantly alter their body weight (BW), the coefficients of the brain to the body weight were indeed decreased significantly when high-dose nanoparticulate anatase TiO2 and bulk- TiO2 were used (Table 1). As expected, along with increases of nanoparticulate anatase TiO2 dosage used, the accumulation of titanium in the brain was elevated; and we also observed significant pathological changes in the brains

Conclusion

Nanoparticulate anatase TiO2 injected at the mouse abdominal cavity appear able to migrate anteriorly into the brain, then cause the oxidative stress and injury of the brain, and subsequently disturb the normal metabolism of neurochemicals. Oxidative stress we observed showed that the effects of nanoparticulate anatase TiO2 on the mouse brain occurred at the cellular and molecular levels. Our findings imply that the regulation of both ROS and signaling molecules (NO) plays a role in

Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant No. 30901218) and by the Medical Development Foundation of Soochow University of China (grant No. EE120701) and by the National Bringing New Ideas Foundation of Student of China (grant No. 57315427, 57315927).

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    Linglan Ma, Jie Liu, Na Li and Jue Wang contributed equally to this work.

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