Short CommunicationA protocol for the differentiation of human embryonic stem cells into dopaminergic neurons using only chemically defined human additives: Studies in vitro and in vivo
Section snippets
Acknowledgments
All work on NIH approved cell lines was supported by NIH NS32519, NS43309, NS48315 and PA. State SAP4100026302 C.U.R.E. All work on non-NIH approved lines was supported through the kind generosity of the Farber Institute for Neurosciences at Thomas Jefferson University.
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Cell therapy for parkinson’s disease
2019, Comprehensive BiotechnologyPluripotent stem cell-based therapy for Parkinson's disease: Current status and future prospects
2018, Progress in NeurobiologyCitation Excerpt :The majority of knowledge obtained from mESCs was readily adapted to hESCs, leading to the development of several protocols for efficient production of mDA cell populations. Initially, the stromal co-culture method was widely used (Brederlau et al., 2006; Buytaert-Hoefen et al., 2004; Cooper et al., 2010; Iacovitti et al., 2007; Kim et al., 2010a; Ko et al., 2007; Morizane et al., 2010; Park et al., 2005; Perrier et al., 2004; Sonntag et al., 2007; Yan et al., 2005; Yang et al., 2008; Zeng et al., 2004), but other methods, including the production of neural cell suspensions, were also introduced (Ben-Hur et al., 2004; Cho et al., 2008; Doi et al., 2012; Schulz et al., 2004). As shown in Table 1, all these protocols utilized DA-specific morphogens and growth factors such as SHH, FGF8, BDNF, GDNF, and/or dbcAMP, and in addition to stromal cells, fetal midbrain astrocytes were also used in co-cultures to enhance mDA differentiation of hESCs (Roy et al., 2006; Song et al., 2018; Wagner et al., 1999).
A simple method for differentiation of H9 cells into neuroectoderm
2015, Tissue and CellPatch-clamp recordings and calcium imaging followed by single-cell PCR reveal the developmental profile of 13 genes in iPSC-derived human neurons
2014, Stem Cell ResearchCitation Excerpt :hESC-H9 colonies were obtained from the University of Connecticut Stem Cell Core. Stem cell lines were differentiated using a five stage protocol (Fig. 1A) with defined media constituents (Belinsky et al., 2011; Iacovitti et al., 2007). This protocol does not use typical brain region morphogens in Stages 4–5 (besides those present in B27), and so is associated with the default production of forebrain neurons (Zeng et al., 2010).