Letter to the EditorM22 based (manual) ELISA for TSH-receptor antibody (TRAb) measurement is more sensitive than 2nd generation TRAb assays
References (5)
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Technical evaluation of the first fully automated assay for the detection TSH receptor autoantibodies
Clin Chim Acta
(2008) - et al.
Comparison of M22-based ELISA and human-TSH-receptor-based luminescence assay for the measurement of thyrotropin receptor antibodies in patients with thyroid diseases
Horm Metab Res
(2008)
Cited by (22)
Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases
2015, Clinica Chimica ActaCitation Excerpt :The ASGPR Ab ELISA has got an intra-assay variability of 4.5% and an inter-assay variability of 9.5% for a positive serum with a BI of 1.2 [11]. The functional assay sensitivity determined as described elsewhere [28] equals a BI of 0.33. For the detection of AIH-related Abs by conventional IIF, commercially available assays employing HEp-2 cells and rodent kidney/liver/stomach tissue sections as autoantigenic substrates were used according to the recommendations of the manufacturers (Euroimmun, Lübeck, Germany, GA Generic Assays) and the recommendations made by the serology committee of the International Autoimmune Hepatitis Group (IAIHG) [29,30].
Autoantibodies to GP2, the major zymogen granule membrane glycoprotein, are new markers in Crohn's disease
2011, Clinica Chimica ActaCitation Excerpt :Intra- and inter-assay coefficients of variations (CVs) were calculated. The functional assay sensitivity (FAS) for anti-GP2 antibody detection was determined as described previously [23]. In view of published studies reporting the frequent co-occurrence of PAB and antibodies against the mannan of S. cerevisiae (ASCA) and in order to determine whether anti-GP2 antibodies, the major autoantigen of PAB, is associated with ASCA, serum samples from patients and controls were also tested for this anti-microbial reactivity.
Clinical review about TRAb assay's history
2010, Autoimmunity ReviewsCitation Excerpt :TRAb are detected by their ability to inhibit M22 binding [42]. Functional assay sensitivity of the “full version” M22 based TRAb assay was found to be 0.3 IU/L [43], strictly calibrated to NIBSC 90/672 [41,44]. In contrast, the fas of the “short version” was at 1.8 IU/L (NIBSC 90/672) lower due to its shortened assay procedure [45].