Cell Reports
Volume 3, Issue 1, 31 January 2013, Pages 70-78
Journal home page for Cell Reports

Report
Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis

https://doi.org/10.1016/j.celrep.2012.12.017Get rights and content
Under a Creative Commons license
open access

Summary

Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis.

Highlights

Lgr5+ cells regenerate mammary epithelium more effectively than other basal cells ► Single Lgr5+ cells are exceptionally efficient in reconstituting mammary glands ► Depletion of Lgr5+ cells leads to impaired mammary gland pubertal development ► Lgr5+ cells are sufficient and necessary for postnatal mammary gland organogenesis

Cited by (0)

4

These authors contributed equally to this work