Current Biology
Volume 15, Issue 10, 24 May 2005, Pages 968-973
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NRG1, a CC-NB-LRR Protein, together with N, a TIR-NB-LRR Protein, Mediates Resistance against Tobacco Mosaic Virus

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Summary

In animals and plants, innate immunity is regulated by nucleotide binding domain and leucine-rich repeat (NB-LRR) proteins that mediate pathogen recognition and that activate host-cell defense responses [1, 2]. Plant NB-LRR proteins, referred to as R proteins, have amino-terminal domains that contain a coiled coil (CC) or that share similarity with animal Toll and interleukin 1 receptors (TIR) [3]. To investigate R protein function, we are using the TIR-NB-LRR protein N that mediates resistance against tobacco mosaic virus (TMV) [4] through recognition of the TMV p50 protein. Here, we describe N requirement gene 1 (NRG1), a novel N-resistance component that was identified by a virus-induced gene silencing (VIGS) screen of a cDNA library [5]. Surprisingly, NRG1 encodes an NB-LRR type R protein that, in contrast to N, contains a CC rather than a TIR domain. Our findings support emerging evidence that many disease-resistance pathways each recruit more than a single NB-LRR protein. The results also indicate that, in addition to the previously recognized role in elicitor recognition, NB-LRR proteins may also function in downstream signaling pathways.

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1

Present address: UMR, Santé de la Vigne et Qualité du Vin, Institut National de la Recherche Agronomique/Université Louis Pasteur, Laboratoire de Génétique et d'Amélioration de la Vigne, 28 Rue de Herrlisheim BP507, F-68021 Colmar Cedex, France.

2

Present address: Department of Plant Pathology, University of California, Riverside, 900 University Avenue, Riverside, California 92521.