Original ContributionPostischemic hyperoxia reduces hippocampal pyruvate dehydrogenase activity
Section snippets
Materials
All chemicals and reagents were purchased from Sigma–Aldrich unless otherwise stated.
Canine cardiac arrest model
Animal experimentation was performed according to the guidelines of the Institutional Animal Use and Care Committee of the University of Maryland, Baltimore. The animal model used for these studies has been used extensively by us and others to study global cerebral ischemia and reperfusion [15], [16], [17], [18], [19], [20]. Adult purebred female beagles weighing 10–15 kg were anesthetized initially with an
Canine cardiac arrest and resuscitation
Whereas there were no significant differences in baseline physiologic parameters, including pH, temperature, pO2, and pCO2 between animal groups, the pO2 at 30 min reperfusion for hyperoxic animals (467.6 ± 29.7 mm Hg, SE, n = 8) was significantly greater (p < 0.001) than the pO2 of normoxic resuscitated animals (89.2 ± 4.3 mm Hg, SE, n = 8).
Postischemic hippocampal and frontal cortex PDHC enzyme activity
The values for brain tissue maximal PDHC specific activity obtained in these experiments are within the range of previously published results for canine
Discussion
The most important new observations reported in this study are that hyperoxic resuscitation after cardiac arrest reduces hippocampal PDHC enzyme activity and elevates 3-nitrotyrosine immunoreactivity compared to values obtained with sham-operated control animals or those resuscitated using normal arterial O2 levels. The finding that hippocampal 3-nitrotyrosine immunoreactivity measured by ELISA is elevated after hyperoxic but not normoxic resuscitation is consistent with results we obtained
Acknowledgments
The authors thank Ms. Kyni Jones and Dr. Cynthia Cotta-Cumba for expert technical assistance. This work was supported by NIH NS34152, NS049425, NS050653, and AHA 0215331U and U.S. Army DAMD 17-03-1-0745.
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