Original ContributionThe relationship between dose of vitamin E and suppression of oxidative stress in humans
Section snippets
Participants
These studies were approved by the Vanderbilt University Institutional Review Board and all participants gave informed consent. Individuals with polygenic hypercholesterolemia were studied. Men and women who had a total serum cholesterol level higher than 200 mg/dl at the initial screening evaluation were selected for entry into the study. Subjects with any known disease except for minor nonsystemic ailments were excluded, as were individuals who smoked or were taking multivitamins or other
Time-course study
The time course of the effect of daily administration of 3200 IU/day of vitamin E on reduction of plasma concentrations of F2-isoprostanes is shown in Fig. 1. Repeated measures of trend analysis showed a significant overall time effect on suppression of plasma concentrations of F2-isoprostanes by vitamin E (p < 0.001). A significant reduction from mean plasma concentrations of F2-isoprostanes at week 0 (61.7 ± 6.5 pg/ml) did not occur until after 16 weeks of vitamin E supplementation (36.3 ±
Discussion
This study has defined the clinical pharmacology of vitamin E in humans with increased oxidative stress associated with polygenic hypercholesterolemia. The goal of these experiments was to obtain information regarding the relationship between dose of vitamin E and suppression of oxidative stress in humans and the duration of treatment required to achieve this effect. This information is essential for the interpretation of clinical studies that have been performed exploring the ability of
Acknowledgments
This work was supported by NIH Grants GM42056 (MERIT Award to L.J.R.); HL65709 and HL57986 (to S.F.); HL58427 (to M.F.L.); DK48831, GM15431, and ES13125 (to J.M.); and DK26657.
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2020, JACC: Basic to Translational ScienceCitation Excerpt :Contemporary antioxidants have been largely ineffective in such diseases, including AF. However, therapeutically used doses of antioxidants such as vitamin E and fish oil are not effective to reduce in vivo measures of oxidative injury (e.g., F2-isoprostanes, widely used sensitive markers of oxidative stress) (57–59). Dicarbonyl scavengers represent an alternative strategy to leave ROS generation intact, but to rapidly scavenge reactive lipid mediators as they form, rendering them inactive, so that they cannot interact with their biological targets.