Original ContributionProtection of human cultured cells against oxidative stress by Rhodiola rosea without activation of antioxidant defenses
Section snippets
Reagents
All reagents except those listed below were obtained from Sigma–Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin/streptomycin, trypsin, and cumene hydroperoxide were obtained from Thermo Fisher Scientific (Waltham, MA, USA) for the 143B cell culture study. Versene and DMEM were obtained from Invitrogen, and FBS from HyClone, for the IMR-32 cell culture study.
Cells and cell culture
Human osteosarcoma-derived 143B, human
Relative toxicity of R. rosea versus ascorbate
Our initial aim was to estimate the relative toxicity of the R. rosea extract compared to a commonly used antioxidant, ascorbate (vitamin C), and also to identify an appropriate dose that offers beneficial effects with no toxicity. Our findings in 143B cells (Fig. 2) demonstrated that the R. rosea extract is comparable in toxicity to ascorbate, or may be less toxic (P < 0.001) at 10 μg/ml, in our experimental system. It should also be noted that the putative active compounds (rosin, rosavin, and
Discussion
R. rosea belongs to a growing list of herbal remedies that are attracting attention in the modern medical research community [24], [25]. It is a member of a class of extracts containing compounds known as adaptogens, which are natural herbal extracts that are capable of protecting an individual from stress, anxiety, and fatigue [1], [26], [27], [28]. But, despite its demonstrative benefits in both human trials and animal studies, its molecular mode of action is not known. Twenty-eight
Acknowledgments
We thank Drs. Young Kwon and Douglas C. Wallace for the use of their laboratory space and equipment and Professor Jeffrey A. Johnson for the ARE–luciferase construct.
References (34)
- et al.
Phyto-adaptogens protect against environmental stress-induced death of embryos from the freshwater snail Lymnaea stagnalis
Phytomedicine
(2000) - et al.
Active-oxygen scavenging activity of traditional nourishing-tonic herbal medicines and active constituents of Rhodiola sacra
J. Ethnopharmacol.
(1999) Hormesis defined
Ageing Res. Rev.
(2008)Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays
J. Immunol. Methods
(1983)- et al.
spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase
J. Biol. Chem.
(1952) Glutathione peroxidase and hydroperoxides
Methods Enzymol.
(1978)- et al.
Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid)
Anal. Biochem.
(1988) - et al.
Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples
Anal. Biochem.
(1990) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine
Anal. Biochem.
(1980)- et al.
Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman's reagent
Anal. Biochem.
(1986)
Activation of antioxidant/electrophile-responsive elements in IMR-32 human neuroblastoma cells
Arch. Biochem. Biophys.
Phosphatidylinositol 3-kinase, not extracellular signal-regulated kinase, regulates activation of the antioxidant-responsive element in IMR-32 human neuroblastoma cells
J. Biol. Chem.
The BBC survey of complementary medicine use in the UK
Complement. Ther. Med.
Hormesis in aging
Ageing Res. Rev.
S. Rhodiola rosea: a possible plant adaptogen
Altern. Med. Rev.
The role of humoral factors of regenerating liver in the development of experimental tumors and the effect of Rhodiola rosea extract on this process
Neoplasma
Antioxidative effects of Cinnamomi cassiae and Rhodiola rosea extracts in liver of diabetic mice
BioFactors
Cited by (54)
Rhodiola rosea and salidroside commonly induce hormesis, with particular focus on longevity and neuroprotection
2023, Chemico-Biological InteractionsAnti-senescence effects of Rhodiola crenulate extracts on LO<inf>2</inf> cells and bioactive compounds
2023, Journal of EthnopharmacologyGender-specific effects of pro-longevity interventions in Drosophila
2023, Mechanisms of Ageing and DevelopmentCitation Excerpt :Consumption of food containing R. rosea extract at a concentration of 25 mg/ml extended both the mean lifespan of flies (24% in both sexes) and the maximum lifespan (16% in males and 31% in females) (Schriner et al., 2009). A decrease in endogenous superoxide levels may be a possible molecular mechanism for the longevity effects of this root extract (Schriner et al., 2009). The effect of R. rosea on lifespan in Drosophila was highly dependent on dietary composition (Gospodaryov et al., 2013).
Rhodiola imbricata
2021, Himalayan Medicinal Plants: Advances in Botany, Production & Research