Original ContributionAge-related changes in brain mitochondrial DNA deletion and oxidative stress are differentially modulated by dietary fat type and coenzyme Q10
Section snippets
Chemicals
All the chemical products and solvents, of the highest grade available, were acquired from Sigma (St. Louis, MO, USA) and Merck (Darmstadt, Germany).
Animals and diets
A total of 64 male Wistar rats (Rattus norvegicus) initially weighing 80–90 g were housed four to a cage and maintained in a 12-h light/12-h dark cycle, with free access to food and water. The rats were randomly assigned to four experimental groups and fed from weaning until 24 months of age a semisynthetic and isoenergetic diet according to the
Body weight, brain weight, and food intake
The body weights of the animals were similar for all groups at 6 and at 24 months (data not shown). Aging led to similar increases in body weight for all groups. No differences concerning brain weight were found between groups or by age (data not shown). The brain-to-body weight ratios (data not shown) were similar for all groups, being lower at 24 months. No differences concerning food intake were found between groups or in relation to age.
Mitochondrial fatty-acid profile
When a nutritional intervention is conducted, the
Discussion
Studies of dietary fat (mainly long-chain PUFAs from the (n-3) family) effects on neurobiology and neurodegeneration have a long history [43]. There are important links between fatty acid unsaturation and aging: it has been previously reported that n-6 PUFA-rich diets lead to higher levels of lipid peroxidation and DNA double-strand breaks in rat tissues and blood during aging, compared with MUFA-based diets [9], [10], [11], [12]. Moreover, it has been described that long-lived animals have
Acknowledgments
This study was supported in part by I + D grants from the Spanish Ministry of Education and Science (1FD97-0457-c02-01, BFU2009-11879/BFI, AGL2006-12433, and AGL2008-01057), the Spanish Ministry of Health (Red de Envejecimiento y Fragilidad RD06/0013/0012, PI04/0355, and PI05/2214), and the Autonomous Government of Andalusia (AGR832) and Catalonia (2009SGR735). We thank David Argiles for excellent technical assistance. We are also grateful to Dr. Ricardo Ramos, from the Genomic Facility of the
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