Diversification of fasting regulated transcription in a cluster of duplicated nuclear hormone receptors in C. elegans
Section snippets
Rhabditidae genomes encode far more NHRs than insects or mammals
While the Drosophila genome contains only 18 nhr genes and the human genome contains 48 such genes, sequence homology identifies more that 284 nhrs in the Caenorhabditis elegans genome (Antebi, 2006, Haerty et al., 2008, King-Jones and Thummel, 2005, Sluder et al., 1999), 232 in Caenorhabditis briggsae (reported by Haerty et al. (2008)) and 256 in Caenorhabditis remanei (Haerty et al., 2008). Of the 284 nhr genes in C. elegans, only 13 (Robinson-Rechavi et al., 2003, Robinson-Rechavi et al.,
Worm cultures and lines
Caenorhabditis elegans and C. briggsae worms were obtained from C. elegans Stock Center and cultures were maintained as described (Brenner, 1974). Stock cultures were frozen in liquid nitrogen and fresh cultures were started from frozen stocks when necessary. Larvae were grown on nematode growth medium (NGM) plates or on 2% agarose-capped plates and fed the OP50 bacterial strain of E. coli (Brenner, 1974).
Transgenic lines were prepared as described (Mello and Fire, 1995) from N2 wild type
Acknowledgments
We thank Dr. A. Fire for vectors pPD95.67, L4440 and HT115 cells host. The work was supported by Grant 304/08/0970, Grant 304/07/0529 from the Czech Science Foundation and by Grant 0021620806 from the Ministry of Education, Youth and Sports of the Czech Republic. M.W.K. was supported by the Intramural Research Program of the National Institutes of Health, USA.
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