Original articleExperimental endoscopyBiochromoendoscopy: molecular imaging with capsule endoscopy for detection of adenomas of the GI tract
Section snippets
Animal models
A protocol for animal use in this study was approved by the University of North Carolina Institutional Animal Care and Use Committee. Mice carrying an inactivating Min mutation in the adenomatous polyposis coli gene (ApcMin/+ mice) were used as a well-characterized model of premalignant adenomatous colonic polyps.18ApcMin/+ mice on a C57BL6 background and normal wild-type (WT) littermates were purchased from Jackson Laboratories (Bar Harbor, Me) and expanded in our facility. For benign lesions
NIRF-integrated CE detects different intensity NIRF signals
Activated Prosense 680 (AP) was obtained by mixing 10 μL of 10 mmol/L Prosense 680 and 20 μL of 5% trypsin in 70 μL of PBS (pH 7.4) at room temperature. Different concentrations were achieved by 1:5 and 1:10 dilution in PBS. The NIRF-integrated CE was capable of detecting NIRF signal intensity across this 10-fold probe concentration difference (Fig. 3). This provided initial proof of principle that the modified CE system can detect NIRF.
Detection of adenomatous polyps in ApcMin/+ mice by modified CE
A white light image of a segment of Prosense 680 injected
Discussion
We have demonstrated proof of principle for biochromoendoscopy by integrating “molecular beacons” with NIRF imaging and conventional CE. Our modified CE was capable of detecting NIRF signals emitted from adenomatous polyps in intestines of ApcMin/+ mice injected with a cathepsin B–activatable probe. Importantly, such NIRF signals were specific to the precancerous adenomatous polyps. Benign growths such as hyperplastic polyps or lymphoid aggregates found in similarly treated SOCS2-HT/GH-TG or
Acknowledgments
We thank Dr Yijing Chen (Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC) for technical assistance.
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Presented at Digestive Disease Week, Washington DC, May 23, 2007 (Gastrointest Endosc 2007;65:AB350).