Original contributionChromosomal aberrations in esophageal squamous cell carcinoma among chinese: gain of 12p predicts poor prognosis after surgery
Section snippets
Primary tumor specimens and DNA extraction
Fresh specimens of primary tumor were collected after esophagectomy and stored at −80°C until DNA extraction. Specimens collected from 1994 to 1997 at Queen Mary Hospital, Hong Kong were included in this study. All patients were treated by surgery only, with esophagectomy and lymph node dissection; patients with preoperative or adjuvant postoperative chemotherapy or radiotherapy given were excluded. A total of 60 specimens were analyzed, all from ethnic Chinese patients. All specimens were
Results
Fifty-two of the 60 (86.7%) primary ESCCs exhibited chromosomal imbalances. Fig 1 shows the summarized CGH results. Fig 2 shows an example of the CGH ratio profiles. On average, there were 10.5 aberrations per tumor. The most frequently detected sites of DNA gains were 3q (35 cases; 67.3%), 8q (30 cases; 57.7%), and 5p (27 cases; 51.9%), with the minimal region of overlap in 3q26.2-qter, 8q23-qter, and 5pter-p14, respectively. Other chromosomal sites commonly demonstrating DNA gains included
Discussion
ESCC is a disease with poor prognosis; the overall 5-year survival after surgery alone is 5% to 20%. Studies have reported improved survival with combined modality treatment compared with radiotherapy or surgery alone.10, 11 However, more treatment brings additional toxicity and morbidity. If other markers besides stage can predict for prognosis, patients who are predicted to be at high risk for relapse after surgery alone can be selected for combined modality treatment. Those who would do well
References (29)
- et al.
Chromosome 4 deletions are frequent in invasive cervical cancer and differ between histologic variants
Gynecol Oncol
(2000) - et al.
Mapping of genetic deletions on the long arm of chromosome 4 in human esophageal adenocarcinomas
Am J Pathol
(1999) - et al.
Cyclin D2 overexpression and lack of p27 correlate positively and cyclin E inversely with a poor prognosis in gastric cancer cases
Am J Pathol
(2000) - et al.
Estimates of the worldwide mortality from 25 cancers in 1990
Int J Cancer
(1999) - et al.
Cyclin D1 expression is useful as a prognostic indicator for advanced esophageal carcinomas, but not for superficial tumors
Dig Dis Sci
(2000) - et al.
Significance of int-2/hst-1 coamplification as a prognostic factor in patients with esophageal squamous carcinoma
Cancer Res
(1994) - et al.
Prognostic significance of biologic factors in squamous cell carcinoma of the esophagus
Cancer
(1999) - et al.
Modern pathology. Prognostic parameters in squamous cell carcinoma of the esophagus: Recent results
Cancer Res
(2000) - et al.
Histochemical study of vascular endothelial growth factor in squamous cell carcinoma of the esophagus
Hepatogastroenterology
(1999) - et al.
p150 expression and its prognostic value in squamous cell carcinoma of the esophagus
Int J Cancer
(1999)
Purification of nucleic acids from eukaryotic cells
Optimizing comparative genomic hybridization for analysis of DNA copy sequence number changes in solid tumors
Genes Chromosomes Cancer
Combined chemotherapy and radiotherapy compared with radiotherapy alone in patients with cancer of the esophagus
N Engl J Med
A comparison of multimodal therapy and surgery for esophageal adenocarcinoma
N Engl J Med
Cited by (57)
Current perspectives of mi-RNA in oesophageal adenocarcinoma: Roles in predicting carcinogenesis, progression and values in clinical management
2015, Experimental and Molecular PathologyCitation Excerpt :Oesophageal squamous cell carcinoma is more commonly noted. Most of the molecular works have been performed in oesophageal squamous cell carcinoma as oesophageal squamous cell carcinoma is more common in areas with high prevalence of oesophageal cancer (Lam, 2000; Chan et al., 2006, 2013; Pak et al., 2009; Tang et al., 2007a,b; Law et al., 2007; Chung et al., 2007; Wong et al., 2006; Fatima et al., 2006; Kwong et al., 2004). On the other hand, oesophageal adenocarcinoma is often noted in the Western populations with the aetiology linked to Barrett oesophagus (Kumarasinghe et al., 2014).
The roles of JK-1 (FAM134B) expressions in colorectal cancer
2014, Experimental Cell ResearchJK1 (FAM134B) gene and colorectal cancer: A pilot study on the gene copy number alterations and correlations with clinicopathological parameters
2014, Experimental and Molecular PathologyCitation Excerpt :This suggests that JK-1 (FAM134B) is potentially one of these tumor suppressor genes. Using comparative genomic hybridization in 52 cases of esophageal squamous cell carcinoma, we noted that 52% of the cancers showed gain in 5p (Kwong et al., 2004). This study was however conducted on esophageal carcinoma which may have different molecular pathways of carcinogenesis (Maehara et al., 2010).
A pan-cancer analysis reveals CHD1L as a prognostic and immunological biomarker in several human cancers
2023, Frontiers in Molecular Biosciences