Reviews and feature articleUnique and overlapping gene expression patterns driven by IL-4 and IL-13 in the mouse lung
Section snippets
Animals
Four-week-old female BALB/c mice were purchased from Jackson Laboratories (Bar Harbor, Me). All mice were housed under laminar flow hoods in an environmentally controlled specific pathogen-free animal facility. The studies reported conformed to the principles for laboratory animal research, as outlined by the Animal Care and Use Committee of Cincinnati Children's Hospital Medical Center.
Allergen and cytokine treatment protocols
Mice were sensitized by means of intraperitoneal injection of 150 μg of endotoxin-free ragweed pollen (RWP)
Comparison of allergen and TH2 cytokine–induced gene expression patterns
Initial experiments compared gene expression in whole lungs isolated from mice challenged with PBS, RWP, or HDM or with rIL-4, rIL-13, or rIL-4 plus rIL-13. Of the approximate 19,207 unique genes and 7,600 expressed sequence tags represented by the 45,000 probes on the array set, expression of 1,813 gene transcripts was found to be significantly different in the lungs of allergen- or cytokine-treated lungs compared with that seen in their corresponding control groups. Hierarchic cluster
Discussion
The present study used Affymetrix microarray technology to comprehensively profile gene expression in the allergic lung to gain insight into the molecular mechanisms underlying the pathogenesis of asthma and to identify novel targets for therapeutic development. Our specific objectives were to define the patterns of expression associated with allergen challenge in the mouse lung and determine the relative contribution of IL-4 and IL-13 to this pattern. To this end, we identified an “asthma
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Cited by (0)
Supported by PO1 HL076383 to M.W-K. and F.D.F.; HL67736-08 (M.W-K.) and AI052099 (F.D.F.); and the CONICIT and Universidad Centroccidental Lisandro Alvarado (Venezuela) to J.S. C.C.L. has received research support from the National Institute of Allergy and Infectious Diseases.
Disclosure of potential conflict of interest: F. D. Finkelman is a consultant for Abbott. The rest of the authors have declared that they have no conflict of interest.