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Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances

https://doi.org/10.1016/j.jbbm.2006.08.008Get rights and content

Abstract

To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted.

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Acknowledgement

This study was supported in part by a grant from Japan Foundation for Aging and Health.

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