Original articleSerum levels of MMP-3 and cathepsin K in patients with ankylosing spondylitis: Effect of TNFα antagonist therapy☆
Section snippets
Methods
We prospectively included outpatients who met modified New York criteria for AS [11]. For each patient, we recorded age, disease duration, main features, and treatment. To assess disease activity, we used the validated French version of the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) [12] and we measured the erythrocyte sedimentation rate (ESR) and the serum C-reactive protein level (CRP). The control group was composed of healthy volunteers who had no known diseases and were
Results
We included 23 patients with AS and 21 age-matched healthy controls. The patients were 18 men and 5 women with a mean disease duration of 10.1 ± 1.3 years and predominant involvement of the axial skeleton (21/23 patients). Most of the patients (19/23, 82%) were HLA-B27-positive. The BASDAI was 44.1 ± 4.1, CRP was 22.3 ± 4.7 mg/L, and ESR was 27.8 ± 5.3 mm/h. None of the patients were taking glucocorticoids or immunomodulating drugs. Nonmusculoskeletal manifestations included inflammatory bowel disease in
Discussion
In a group of patients with active AS (BASDAI > 40) chiefly involving the axial skeleton, serum MMP-3 levels were significantly elevated compared to healthy controls. None of our patients was taking immunosuppressive drugs or glucocorticoids, which may increase MMP-3 levels [13]. Similarly, in 17 patients with axial AS and 16 with axial and appendicular AS, serum MMP-3 levels were elevated compared to healthy controls [14]. MMP-3 production indicated the presence of peripheral synovitis: MMP-3
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2016, Autoimmunity ReviewsCitation Excerpt :Cathepsin K, an enzyme expressed predominantly in osteoclasts triggered by inflammatory cytokines, is involved in bone remodelling and resorption. Although systemic levels of cathepsin K in patients with AS were similar to those in healthy controls [49], there was a strong expression of cathepsin K in mononuclear cells, fibroblast-like cells and cells attached to bone in patients with AS in contrast to those with degenerative disc disease [50]. Osteopontin is a secreted protein expressed by various cells including inflammatory cells, chondrocytes or cells involved in bone metabolism, such as osteoclasts and osteoblasts.
The development and characterization of an ELISA specifically detecting the active form of cathepsin K
2013, Clinical BiochemistryCitation Excerpt :It has been indicated that increased levels of cathepsin K are associated with increased fracture risk in the hip [18], findings which correlate with the observed reduction in cathepsin K levels by treatment with the bisphosphonate alendronate [19]. Furthermore, elevated cathepsin K levels are found in rheumatoid arthritis [20], Paget's disease [16], and ankylosing spondylitis [21], all diseases characterized by excessive bone resorption. However, total cathepsin K does not indicate the activity of the enzyme, and since active cathepsin K is the target of inhibitor development for therapeutic application, we focused on developing an ELISA-based assay specifically detecting active cathepsin K in serum samples.
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This study was funded by the Association Franc-Comtoise pour la Formation, la Recherche et l'Enseignement en Rhumatologie.