Original articleCorticotropin-releasing hormone and urocortin expression in peripheral blood cells from experimentally infected cattle with Mycobacterium avium subsp. paratuberculosis
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Keywords
Urocortin
Corticotrophin-releasing hormone
Mycobacterium avium subspecies paratuberculosis
Paratuberculosis
Neuropeptide
Bovine
Abbreviations
CRH
corticotropin-releasing hormone
ELISA
enzyme-linked immunosorbent assay
GSP
gene specific primer
HPA
hypothalamic-pituitary-adrenal
IFN-γ
interferon gamma
IL
interleukin
LPS
lipopolysacharide
Map
Mycobacterium avium subspecies paratuberculosis
NGSP
nested gene specific primer
NUP
nested universal primer
PBMC
peripheral blood mononuclear cell
PPD
purified protein derivative
RACE
rapid amplification of cDNA ends
RT-PCR
reverse transcription-polymerase chain reaction
TNF-α
tumor necrosis factor alpha
UCN
urocortin
UPM
universal primer mix
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