Novel lethal mouse mutants produced in balancer chromosome screens
Section snippets
Results and discussion
Mutagenesis screens have been used in the mouse since the 1950s. Originally, screens were performed to determine the efficacy of radiation at inducing mutations in seven visible loci – called the “Specific Locus Test” (SLT) (reviewed in Davis and Justice, 1998). Using the SLT, the chemical ENU was identified as a potent mutagen to induce point mutations in spermatogonial stem cells (Hitotsumachi et al., 1985). ENU screens have been used to identify recessive developmental defects in haploid
Mutagenesis screens
The design of the balancer chromosomes and the mutagenesis screens have been described in detail (Zheng et al., 1999, Kile et al., 2003, Hentges and Justice, 2004, Nishijima et al., 2003). For chromosome 4, we classified a line as lethal if no tester animals were recovered in a cohort of 30 carriers or homozygous balancer mice. For chromosome 11, we classified a line as lethal if no tester animals were recovered from that line at weaning in a cohort of 24 carriers. Lines where tester animals
Acknowledgements
The authors thank Colleen Viator and Maritess Alviento for superb technical assistance. This research was funded by U01 HD39372 to M.J.J. and F32 HD42436 to K.E.H.
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