Elsevier

Neuroscience Letters

Volume 419, Issue 1, 23 May 2007, Pages 49-54
Neuroscience Letters

Effects of engrafted neural stem cells derived from GFP transgenic mice in Parkinson's diseases rats

https://doi.org/10.1016/j.neulet.2007.03.046Get rights and content

Abstract

This study evaluated the therapeutic effect of neural stem cells (NSCs) transplanted into Parkinson's disease (PD) rats. NSCs were identified in vitro, then engrafted into the striatum of the PD rats. The rotational behavior was evaluated 1, 2, 4 and 6 weeks. A significant rotational behavior improvement was observed in PD rats subjected to cell transplantation. Transplanted NSCs not only express Nerve growth factor and Neurotrophin-3 in vitro, but also survive and partly differentiate into tyrosine hydroxylase (TH) positive cells in vivo. The results show that NSCs could be effective for PD treatment and the mechanisms might involve the neurotrophin expression and the neural differentiation.

Section snippets

Acknowledgements

This work was supported by the international collaboration grant of China Chunhui plan (No. 2004-1; No. 2004-2) and Yunnan natural science foundation (No. 2004C0002R).

References (30)

  • M.F. Beal

    Experimental models of Parkinson's disease

    Nat. Rev. Neurosci.

    (2001)
  • T. Ben-Hur et al.

    Transplanted multipotential neural precursor cells migrate into the inflamed white matter in response to experimental autoimmune encephalomyelitis

    Glia

    (2003)
  • T. Ben-Hur et al.

    Transplantation of human embryonic stem cell-derived neural progenitors improves behavioral deficit in Parkinsonian rats

    Stem Cells

    (2004)
  • A. Bjorklund et al.

    Cell replacement therapies for central nervous system disorders

    Nat. Neurosci.

    (2000)
  • L.M. Bjorklund et al.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model

    Proc. Natl. Acad. Sci. U.S.A.

    (2002)
  • Cited by (26)

    • Reduced dopaminergic neuron degeneration and global transcriptional changes in Parkinson's disease mouse brains engrafted with human neural stems during the early disease stage

      2022, Experimental Neurology
      Citation Excerpt :

      Nonetheless, the mixed outcomes of clinical trials provide guidance for developing alternative strategies to improve cell-based therapy for PD. Human neural stem cells (hNSCs) have multiple therapeutic qualities, including production of anti-inflammatory, anti-apoptotic, pro-regenerative, pro-angiogenic, and neurotrophic growth factors (Boese et al., 2020; Boese et al., 2018; Eckert et al., 2015; Hamblin and Lee, 2021; Hamblin et al., 2022; Lee et al., 2007; Lee et al., 2020; Pluchino et al., 2005; Wei et al., 2007; Yasuhara et al., 2006). In rodent models of neonatal hypoxic ischemic injury, transplanted hNSCs extensively migrate to injury sites and some of them differentiate into neurons that integrate with the host neuronal network (Imitola et al., 2004).

    • Dissociation between dopaminergic response and motor behavior following intrastriatal, but not intravenous, transplant of bone marrow mononuclear stem cells in a mouse model of Parkinson's disease

      2017, Behavioural Brain Research
      Citation Excerpt :

      Thus, other alternatives have been investigated, and one promising possibility is stem cell transplant. Many pre-clinical and clinical studies have used fetal [8–15], neural [16,17] or embryonic [18,19] cells to treat PD and have shown optimistic results. Ethical and religious concerns, however, make the use of these cellular sources inappropriate in clinical practice [20].

    • Stem cell therapy for amyotrophic lateral sclerosis

      2015, Cell Regeneration
      Citation Excerpt :

      The integration ability and prospective therapeutic efficacy of human neural stem cells (hNSC) has been demonstrated in rodent models of neurological diseases [20–23]. Apart from regenerating lost neuronal cells, NSCs can also improve the functional outcomes of rats through auxiliary mechanisms, such as neurotrophism [24–26] and immunosuppression [27–29]. A number of studies have demonstrated that NSC therapy had beneficial effects on ALS rats [17, 30].

    • BDNF expression with functional improvement in transected spinal cord treated with neural stem cells in adult rats

      2013, Neuropeptides
      Citation Excerpt :

      All efforts were made to minimize the number of animals and their suffering during the experiments. NSC was obtained from the hippocampal tissue of green fluorescent protein (GFP) transgenic embryonic mouse, then incubated as described previous work (Wei et al., 2007). Briefly, the hippocampal tissues were harvested and washed in D-Hanks’ solution.

    View all citing articles on Scopus
    View full text