NeuroanatomyThe vasopressin 1b receptor is prominent in the hippocampal area CA2 where it is unaffected by restraint stress or adrenalectomy
Section snippets
Animals
Adult C57BL/6J and 129/P3J strains of mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). Avpr1b +/+ and −/− mice of mixed background (C57BL/6J and 129/SvJ) were created as described previously (Wersinger et al., 2002) and bred in our own colony. Six adrenalectomized and six sham-operated C57BL/6 mice were obtained from Taconic (Germantown, NY, USA). Their brains were removed 7 days after surgery. Adult Sprague–Dawley rats were also obtained from Taconic.
All animals were given
Results
The mouse, rat and human Avpr1b riboprobes were tested first on the respective pituitaries and showed good cellular labeling (Fig. 1). The brain distributions of Avpr1b transcripts were the same in C57BL/6J and 129/P3J strains or in the mixed backgrounds that the Avpr1b −/− mice were maintained on, with either the 5′ or 3′ probe. In the brain, labeling of the dorsal hippocampal CA2 pyramidal cells was the highest (Fig. 2) at 20–40 grains per 100 μm2 after a 4 month exposure. In fact, at the
Discussion
The present study revealed expression in the mouse by ISHH in a much more restricted distribution than we had anticipated, being especially prominent in the CA2 field of the hippocampus. This result contrasts with the previous results in the rat using a riboprobe (Vaccari et al., 1998). The discrepancies are not likely due to species differences as the CA2 pyramidal layer was the only region we observed labeled in the rat (Fig. 5). In fact, using a mouse probe analogous to the original rat
Acknowledgments
The authors would like to thank Dr. Éva Mezey and Ildiko Szalayova for assistance with the in situ hybridization histochemistry. Emily Shepard provided excellent technical assistance with the autoradiography and mouse colony maintenance. We thank Dr. Mary Herman for supplying the human pituitary and for neuropathological analysis of the human sections. The critical comments of Dr. Elisabeth Murray are greatly appreciated. This research was supported in part by the NIMH Intramural Research
References (67)
- et al.
Inhibition of the vasopressin-enhancing effect on memory retrieval and relearning by a vasopressin V1 receptor antagonist in mice
Eur J Pharmacol
(1995) - et al.
Evidence that the effects of arginine-8-vasopressin (AVP) on pituitary corticotropin (ACTH) release are mediated by a novel type of receptor
Peptides
(1984) - et al.
Vasopressin and aggression in cross-fostered California mice (Peromyscus californicus) and white-footed mice (Peromyscus leucopus)
Horm Behav
(2001) - et al.
AVP V1b selective antagonist SSR149415 blocks aggressive behaviors in hamsters
Pharmacol Biochem Behav
(2005) - et al.
Chronic intracerebral infusions of vasopressin and vasopressin antagonist modulate behavioral effects of interleukin-1 in rat
Brain Res Bull
(1992) - et al.
Effects of vasopressin on active and passive avoidance behavior
Horm Behav
(1972) - et al.
The origin of the vasopressinergic and oxytocinergic innervation of the rat brain with special reference to the lateral septum
Brain Res
(1983) - et al.
The effects of infusion of arginine vasopressin, oxytocin, or their antagonists into the olfactory bulb upon social recognition responses in male rats
Peptides
(1998) - et al.
V1a receptor knockout mice exhibit impairment of spatial memory in an eight-arm radial maze
Neurosci Lett
(2004) - et al.
The hypothalamic-neurohypophysial system regulates the hypothalamic-pituitary-adrenal axis under stress: an old concept revisited
Front Neuroendocrinol
(2004)
Microdialysis administration of vasopressin and vasopressin antagonists into the septum during pole-jumping behavior in rats
Behav Neural Biol
Inhibition of flank-marking behavior in golden hamsters by microinjection of a vasopressin antagonist into the hypothalamus
Neurosci Lett
Inhibition of vasopressin-stimulated flank marking behavior by V1-receptor antagonists
Eur J Pharmacol
Social behavior functions and related anatomical characteristics of vasotocin/vasopressin systems in vertebrates
Brain Res Brain Res Rev
Mismatches between neurotransmitter and receptor localizations in brain: observations and implications
Neuroscience
Roles of the basolateral amygdala and hippocampus in social recognition in rats
Physiol Behav
The hippocampus as a spatial mapPreliminary evidence from unit activity in the freely-moving rat
Brain Res
Neural correlates of social odor recognition and the representation of individual distinctive social odors within entorhinal cortex and ventral subiculum
Neuroscience
Pattern of neuronal loss in the rat hippocampus following experimental cardiac arrest-induced ischemia
J Neurol Sci
Selective vulnerability of the hippocampus in brain ischemia
Neuroscience
Manipulations of the AVT system shift social status and related courtship and aggressive behavior in the bluehead wrasse
Horm Behav
Three-dimensional analysis of the whole axonal arbors originating from single CA2 pyramidal neurons in the rat hippocampus with the aid of a computer graphic technique
Brain Res
Social motivation is reduced in vasopressin 1b receptor null mice despite normal performance in an olfactory discrimination task
Horm Behav
Role of the hippocampal CA2 region following postischemic hypothermia in gerbil
Brain Res Mol Brain Res
Role of vasopressin and oxytocin in the control of social behavior in Syrian hamsters (Mesocricetus auratus)
Prog Brain Res
Heterogeneous modulation of place cell firing by changes in context
J Neurosci
Neural circuitry controlling vasopressin-stimulated scent marking in Syrian hamsters (Mesocricetus auratus)
J Comp Neurol
Parallel activation of field CA2 and dentate gyrus by synaptically elicited perforant path volleys
Hippocampus
Profound impairment in social recognition and reduction in anxiety-like behavior in vasopressin V1a receptor knockout mice
Neuropsychopharmacology
Distinct substance P- and calretinin-containing projections from the supramammillary area to the hippocampus in rats: a species difference between rats and monkeys
Exp Brain Res
Spatial and temporal expression of alpha- and beta-thyroid hormone receptor mRNAs, including the beta 2-subtype, in the developing mammalian nervous system
J Neurosci
Vasopressin cells in the medial amygdala of the rat project to the lateral septum and ventral hippocampus
J Comp Neurol
Vasopressin, gonadal steroids and social recognition
Prog Brain Res
Cited by (0)
- 1
Present address: Graduate program in Molecular, Cell, Genetics and Development, Yale University, New Haven, CT 06511, USA (J. Li); Department of Psychology, The University at Buffalo, SUNY, Buffalo, NY 14260, USA (S. R. Wersinger).