Cellular neuroscienceDetermination of key aspects of precursor cell proliferation, cell cycle length and kinetics in the adult mouse subgranular zone
Section snippets
Animals
Adult, male C57BL/6J mice (initial weight 23–27 g, 9–11 weeks old; Jackson Laboratories, Bar Harbor, ME, USA) were used. Mice were group housed (maximum five/cage) in a facility approved by the Association for the Assessment and Accreditation of Laboratory Animal Care International (AAALAC) at the University of Texas Southwestern Medical Center, with a 12-h light/dark cycle and with free access to food and water. Mice were acclimated to vivarium conditions for at least 1 week prior to
Dose response of BrdU in adult C57BL/6J mice
To determine the relationship between BrdU dose and BrdU-IR cell counts in the adult mouse SGZ, we performed two separate dose response experiments. In the first experiment (Fig. 1a, experiment I; Fig. 1b), we compared 50 and 150 mg/kg doses (Hayes and Nowakowski 2002, Kronenberg et al 2003, Mandyam et al 2004) with lower (25 mg/kg) and higher (300, 500 mg/kg) doses. In the second experiment (Fig. 1a, experiment II; Fig. 1c), we directly compared 50, 100 and 150 mg/kg doses. In both dose
Discussion
Key aspects of precursor cell division from the adult mouse SGZ are revealed in this study. We identify a dose of BrdU sufficient to saturate the S phase cohort of SGZ precursors in the adult mouse. Using a saturating dose of BrdU, we highlight the tendency of SGZ cells and other proliferative subregions of the dentate gyrus to divide in clusters. Our time course analysis shows the number of cells, clusters, and cells per cluster increasing and then decreasing from 0.25–720 h after BrdU
Acknowledgment
This work was supported by a postdoctoral Ruth Kirschstein National Research Service F32 Award from NIDA (DA018017; C.D.M.), a Ruth Kirschstein National Research Service T32 Award from NIDA (DA007290, G.C.H.), and grants from the National Institute on Drug Abuse (DA016765), National Institute of Mental Health (MH66172), National Institute of Aging and National Alliance for Research on Schizophrenia and Depression to A.J.E. Other acknowledgments: The authors thank Rebecca Norris and Jessica Yee
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2021, Neurobiology of StressCitation Excerpt :According to the time between BrdU administration and sacrifice, the survival of the new-born cells may be studied. CUMS (Vega-Rivera et al., 2020) and chronic social defeat stress (Ito et al., 2017) reduced cells survival, as assessed by BrdU administered at least 4 days before perfusion of animals (Mandyam et al., 2007). Maternal separation followed by a second stressor reduced the ratio of BrdU+/DCX + cells to BrdU + cells, which was used to assess the degree of differentiation of new-born cells in the hippocampus (Han et al., 2019).
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Present address: Committee on the Neurobiology of Addictive Disorders at the Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.