Cell Stem Cell
Volume 3, Issue 5, 6 November 2008, Pages 475-479
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Correspondence
Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation

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Summary

Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.

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These authors contributed equally to this work

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These authors contributed equally to this work