Ischemia-reperfusion injuryExperimental: LiverEffects of Serine Protease Inhibitor and Prostaglandin I2 on Liver Transplantation From Non–Heart-Beating Rat Donors
Section snippets
Materials and Methods
Male Wistar rats were divided into five groups: in group 1, livers were retrieved from heart-beating donors (HB group); in group 2, livers were retrieved from NHBDs that had experienced agonal apnea (NHB group); in group 3, livers were retrieved in the same manner as in the NHBD group but were pretreated with prostaglandin (PG) I2, 33 ng/kg/h for 30 minutes (PGI2 group); in group 4, livers were retrieved in the same manner as in the NHBD group but were pretreated with nafamostat mesilate (NM),
Experiment 1
Portal flow volume in the NHB group decreased significantly compared with that in the HB group; it was maintained as the same level in the NM+PG group as in the HB group (Table). In the NHB group, the values for IL-1β, TNF-α, and TBX2 were significantly greater than those in the HB group. In contrast, in the NM+PG group, the values were significantly lower than those in the NHB group. Histologic analysis revealed well preserved sinusoidal endothelial cells in the NM+PG group, as in the HB group
Discussion
Successful liver transplantation from NHBD or HB marginal donors enlarges the donor source. However, the criteria and standard retrieval procedure of the grafts from those donors have not yet been established.4 In previous studies, we reported that inflammatory cytokines such as TNF-α and IL-1β were increased at reperfusion after cold preservation.2 We also described sinusoidal microcirculatory disturbance as the primary cause of ischemia-reperfusion injury in ΔLT. The microcirculatory
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Understanding “marginal” liver grafts
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Elimination of Kupffer cells and nafamostat mesilate rinse prevent reperfusion injury in liver grafts from agonal non–heart-beating donors
Transplantation
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Cited by (0)
Supported by Grants-in–Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan and from the Ministry of Welfare of Japan, and by a grant from Tohoku University Graduate School of Medicine.