Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
EndodontologyCatonella morbi and Granulicatella adiacens: new species in endodontic infections
Section snippets
Material and methods
Samples collected for previous investigations13, 14 were stored and available for reanalysis in this study. Only single-root teeth from adult patients (ages ranging from 18-60 years), all of them having carious lesions, necrotic pulps, and radiographic evidence of periradicular disease, were included in this study. Selected teeth showed an absence of periodontal pockets deeper than 4 mm. In general, 50 samples of infections of endodontic origin were obtained. According to the forms of
Results
All sample extracts were positive after the first round of PCR amplification by using broad-range 16S rRNA gene primers, indicating that the DNA extraction procedure was adequate, that bacteria were present in all examined samples, and that inhibitors of the PCR reaction were not present. No PCR products were observed in negative controls that used sterile ultrapure water instead of sample.
The specificity of each primer was tested against a panel of representative oral bacteria. The use of each
Discussion
The present study used a 16S rRNA gene-based heminested PCR protocol to detect C morbi and G adiacens in samples taken from endodontic infections associated with different forms of apical periodontitis. Utilization of a nested PCR protocol is justified by the increased sensitivity and specificity of the assay when compared to single PCR. Increased sensitivity is a function of the large total number of cycles used in nested PCR assays. In addition, target DNA is amplified in the first round of
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Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection
2020, iScienceCitation Excerpt :It has been shown that nicotine and its metabolite cotinine, as well as treatment with a cigarette smoke extract, can alter the function of key periodontal pathogens such as P. gingivalis and promote biofilm formation, colonization, and infection (Hanioka et al., 2019). Granulicatella is a known commensal of the human oral microbiome, however, which has been implicated in endodontic infection (Siqueira and Rôças, 2006) and is linked to increased risk of systemic diseases, such as pancreatic cancer related to oral inflammation (Farrell et al., 2012). Cigarette smoking is known to escalate the infection risk of pathogenic or opportunistic bacteria by bringing about physiological and structural changes, increases in bacterial virulence, and immune dysfunction (Bagaitkar et al., 2008).
Role of oral and gut microbiome in nitric oxide-mediated colon motility
2018, Nitric Oxide - Biology and ChemistryCitation Excerpt :The prominent pathogens that are normally found in PD are Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum, Treponema denticola, and Aggregatibacter actinomycetemcomitans [24]. However, certain studies have shown that certain Gram-negative bacteria (Selenomonas [25], Prevotella [26], Treponema [27], Tannerella [28], Haemophilus [29], and Catonella [30]) are particularly enriched in PD. These bacteria release proteolytic enzymes, which in turn release inflammatory signals that result in inflammation of the gingiva, apical migration of junctional epithelium, periodontal pocket formation, and alveolar bone resorption.
Cytokine induction of peripheral blood mononuclear cells by biofilms and biofilm supernatants of Granulicatella and Abiotrophia spp.
2018, Microbial PathogenesisCitation Excerpt :The most frequent infections caused by NVS are endocarditis and sepsis [3,4] but these microorganisms have been implicated also in several others infections such as central nervous system infections [5], sinusitis, otitis media, prostatitis, cholangitis, arthritis [6,7]. The characterization of microbial composition based upon nucleic acid based techniques have revealed that Granulicatella spp. were found elevated in periodontitis [8], caries [9] and endodontic infections [10,11]. In our previous study, coaggregation and biofilm formation of Granulicatella spp. with other oral bacteria such as Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans was observed [12].
On the Oral Microbiome of Oral Potentially Malignant and Malignant Disorders: Dysbiosis, Loss of Diversity, and Pathogens Enrichment
2023, International Journal of Molecular SciencesComplete genome sequence of Granulicatella adiacens KHUD_009 isolated from subgingival biofilm
2023, Korean Journal of Microbiology
This study was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a Brazilian governmental institution.