Elsevier

Virology

Volume 318, Issue 1, 5 January 2004, Pages 24-31
Virology

Rapid Communication
Identification of a novel receptor-like protein kinase that interacts with a geminivirus nuclear shuttle protein

https://doi.org/10.1016/j.virol.2003.09.038Get rights and content
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Abstract

Despite extensive studies in plant virus–host interactions, the molecular mechanisms of geminivirus movement and interactions with host components remain largely unknown. A tomato kinase protein and its soybean homolog were found to interact specifically with the nuclear shuttle protein (NSP) of Tomato golden mosaic virus (TGMV) and Tomato crinkle leaf yellows virus (TCrLYV) through yeast two-hybrid screening and in vitro protein binding assays. These proteins, designated LeNIK (Lycopersicon esculentum NSP-Interacting Kinase) and GmNIK (Glycine max NIK), belong to the LRR-RLK (leucine rich-repeat receptor-like kinase) family that is involved in plant developmental processes and/or resistance response. As such, NIK is structurally organized into characteristic domains, including a serine/threonine kinase domain with a nucleotide binding site at the C-terminal region, an internal transmembrane segment and leucine-rich repeats (LRR) at the N-terminal portion. The potential significance of the NSP–NIK interaction is discussed.

Keywords

Tomato kinase protein
Geminivirus
Nuclear shuttle protein

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