Elsevier

Genomics

Volume 86, Issue 6, December 2005, Pages 739-752
Genomics

Piggy-BACing the human genome: II. A high-resolution, physically anchored, comparative map of the porcine autosomes

https://doi.org/10.1016/j.ygeno.2005.04.010Get rights and content
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Abstract

Using the INRA–Minnesota porcine radiation hybrid panel, we have constructed a human–pig comparative map composed of 2274 loci, including 206 ESTs and 2068 BAC-end sequences, assigned to 34 linkage groups. The average spacing between comparative anchor loci is 1.15 Mb based on human genome sequence coordinates. A total of 51 conserved synteny groups that include 173 conserved segments were identified. This radiation hybrid map has the highest resolution of any porcine map to date and its integration with the porcine linkage map (reported here) will greatly facilitate the positional cloning of genes influencing complex traits of both agricultural and biomedical interest. Additionally, this map will provide a framework for anchoring contigs generated through BAC fingerprinting efforts and assist in the selection of a BAC minimal tiling path and assembly of the first sequence-ready map of the porcine genome.

Keywords

Comparative mapping
Radiation hybrid
BAC
Porcine

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