APSA PaperDiaphragmatic repair through fetal tissue engineering: a comparison between mesenchymal amniocyte– and myoblast-based constructs
Section snippets
Materials and methods
This study was approved by the Harvard Medical School Standing Committee on Animals under protocol no. 03354. For groups 1 and 3, as defined below, this study consisted of post hoc analyses of previously reported animals/grafts [2].
Results
Graft failure was observed in all groups, albeit significantly more frequent in group 3 (5/5, 100%) than in groups 1 and 2 combined (2/9, 22.9%; Fig. 1). There was no statistical difference between groups 1 (1/4, 25%) and 2 (1/5, 20%).
Labeled cells were found in all grafts in groups 1 and 2. However, group 1 grafts appeared to have increased cellularity, as well as higher capillary density by CD31 staining, when compared with groups 2 and 3, although this could not be quantified. As early as 1
Discussion
Over the past 2 decades, CDH mortality rates have decreased dramatically [10], [11]. This increased survival has led to previously unrecognized morbidities, such as recurrence of the diaphragmatic hernia, which is known to be more prevalent after prosthetic repair [12], [13], [14], [15]. Hernia recurrence is believed to stem from normal growth, which leads to traction and eventual detachment of the prosthesis [16]. According to the Congenital Diaphragmatic Hernia Study Group, the most infants
Acknowledgments
The authors thank Ms Tonora Archibald (Department of Pathology, Children's Hospital Boston) for her outstanding histology processing and Mr Jeffrey Pettit (Division of Surgery, Harvard Medical School) for his excellence in surgical laboratory assistance.
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Presented at the 36th Annual Meeting of the American Pediatric Surgical Association, Phoenix, AZ, May 29-June 1, 2005.
S.M.K. was supported by grants from the National Institutes of Health National Research Service Award DK065406-02 and the V.H. Kazanjian Surgical Research Fellowship of the Massachusetts General Hospital.