Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-26T23:36:39.176Z Has data issue: false hasContentIssue false
  • English
  • Français

Serological grouping of Treponema hyodysenteriae

Published online by Cambridge University Press:  15 May 2009

D. J. Hampson ,
J. R. L. Mhoma ,
B. G. Combs  and
J. I. Lee
D. J. Hampson
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia 6150
J. R. L. Mhoma
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia 6150
B. G. Combs
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia 6150
J. I. Lee
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia 6150
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two Australian isolates of Treponema hyodysenteriae which did not fit within the current serological grouping system for these bacteria wrere examined by agarose gel double immunodiffusion tests (AGDP). Isolate Vic1 was serologically unique, and we propose that it becomes the type organism for a new sixth serological group of T. hyodysenteriae (Group F). Isolate Q1 was unusual in that lipopolysaccharide (LPS) extracted from it reacted strongly in AGDP with serum raised against the type organism for serogroup D (A1), and also weakly with serum raised against the type organism for serogroup B (WA1). The nature of this cross-reactivity was examined by using cross-absorbed antisera in AGDP, and by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis.

The pattern of serological cross-reactivity between Q1, A1 and WA1 was complex and was not fully defined, but the isolate Q1 apparently shared low molecular weight ‘serogroup’ LPS antigens with A1, and shared higher molecular weight LPS antigens with WA1. On this basis Q1 was designated as belonging to serogroup D, although it was recommended that this be qualified as D (B) to indicate the presence of weak cross-reactivity with serogroup B. Such serological cross-reactivity may have significance in relation to the development of immunity to T. hyodysenteriae. Isolate Q1 may be a potentially useful organism for vaccine development because of its ability to induce a good serological response to LPS of treponemes from both serogroups D and B.

Type
Special Article
Information
Epidemiology & Infection , Volume 105 , Issue 1 , August 1990 , pp. 79 - 85
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Taylor, DJ, Alexander, TJL. The production of dysentery in swine by feeding cultures containing a spirochaete. Br Vet J 1971; 127: 58–61.CrossRefGoogle ScholarPubMed
2.Harris, DL, Glock, RD, Christensen, CR, Kinyon, JM. Swine dysentery. Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease. Vet Med Small Animal Clinician 1972; 67: 61–4.Google Scholar
3.Baum, DH, Joens, LA. Serotypes of beta haemolytic Treponema hyodysenteriae. Infect Immun 1979; 25: 792–7.Google Scholar
4.Mapother, ME, Joens, LA. New serotypes of Treponema hyodysenteriae. J Clin Microbiol 1985; 22: 161– 4.Google Scholar
5.Lemcke, RM, Bew, J. Antigenic differences among isolates of Treponema hyodysenteriae. Proc Int Pig Vet Soc Cong, Ghent 1984; 183.Google Scholar
6.Hampson, DJ, Mhoma, JRL, Combs, BG, Buddie, JR. Proposed revisions to the serological typing system for Treponema hyodysenteriae. Epidemiol Infect 1989; 102: 75–84.Google Scholar
7.Hampson, DJ, Mhoma, JRL, Combs, BG. Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae. Epidemiol Infect 1989; 103: 275–84.Google Scholar
8.Hunter, D, Wood, T. An evaluation of the API ZYM system as a means of classifying spirochaetes associated with swine dysentery Vet Rec 1979; 104: 383–4.CrossRefGoogle ScholarPubMed
9.Lymbery, AJ, Hampson, DJ, Hopkins, RM, Combs, M, Mhoma, JRL. Multilocus enzyme electrophoresis for identification and typing of Treponema hyodysenteriae and related spirochaetes. Vet Micro 1990, In press.CrossRefGoogle ScholarPubMed