Ribosomal DNA-targeted oligonucleotide probe and PCR assay specific for Fusarium oxysporum
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New foci of strawberry Fusarium wilt in Huelva (Spain) and susceptibility of the most commonly used cultivars
2017, Scientia HorticulturaeCitation Excerpt :Genomic DNA was obtained with a DNA extraction kit (E.Z.N.A. Plant DNA Kit, Omega Bio-tek, Inc., Doraville, USA). A PCR assay for amplification of r-DNA using primers PFO2 and PFO3 established the identity of the isolates as F. oxysporum (Edel et al., 2000). To characterize the isolates, PCR primers FofraF and FofraR were also used.
Current status of the taxonomic position of Fusarium oxysporum formae specialis cubense within the Fusarium oxysporum complex
2011, Infection, Genetics and EvolutionCitation Excerpt :Leslie and Summerell (2006) summarized information for 70 species of Fusarium, and this monographic publication was the first for the genus to integrate morphological and phylogenetic information. The morphological taxonomy of species in the genus Fusarium is based primarily on the structure and abundance of asexual reproductive structures (chlamydospores, phialides, microconidia and macroconidia) and on cultural characteristics (colony texture, color and cultural aroma) (Booth, 1971; Edel et al., 2000; Gordon and Martyn, 1997; Lorens et al., 2006; Nelson, 1991; Nelson et al., 1983). F. oxysporum is characterized mainly by non-septate microconidia formed in false heads on short monophialides, 3-septate macroconidia formed from monophialides on branched conidiophores in sporodochia, and chlamydospores with a smooth or rough wall appearance formed singly or in pairs (Fig. 1).
Screening of chickpea (cicer arietinum L.) varieties against macrophomina phaseolina
2021, Plant Cell Biotechnology and Molecular BiologyEnzyme-Linked Immunosorbent Assay (ELISA) Based Detection of Fusarium Circinatum for Alleviation of Pine Seedlings Wilt
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