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Yeast consensus initiator sequence markedly enhances the synthesis of metallothionein III in Saccharomyces cerevisiae

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Abstract

The conventional translation initiator sequence for heterologous expression of metallothionein III (MTIII) in Saccharomyces cerevisiae was replaced by a yeast consensus initiator region (CAAAAUG). Saccharomyces cerevisiae was transformed with a vector carrying the MTIII gene and the modified initiator sequence had a much higher metal tolerance, Cd accumulation and MTIII production than that with the original initiator sequence, although the level of gene transcripts was similar in both systems. This modification markedly improves MTIII production and may also be used for production of other genes.

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Authors and Affiliations

  1. Institute of Radiation Biology, National Tsing Hua University, Hsinchu, Taiwan

    Sue-Hong Wang & Lih-Yuan Lin

  2. Department of Neurosurgery, Neurological Institute, Veterans General Hospital, Taipei, Republic of China

    Yang-Hsin Shih

Authors
  1. Sue-Hong Wang
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  2. Yang-Hsin Shih
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  3. Lih-Yuan Lin
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Wang, SH., Shih, YH. & Lin, LY. Yeast consensus initiator sequence markedly enhances the synthesis of metallothionein III in Saccharomyces cerevisiae. Biotechnology Letters 20, 9–13 (1998). https://doi.org/10.1023/A:1005362626457

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  • DOI: https://doi.org/10.1023/A:1005362626457

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