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Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment

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Abstract

Activities of several CaMV 35S and FMV 34S promoter derivatives fused to the gusA reporter gene were compared in suspension-cultured Catharanthus roseus cells that were transiently and stably transformed using particle bombardment. Our data demonstrate that the 35S and a deletion derivative of the 34S promoter combined with particle bombardment form useful tools for genetic engineering of C. roseus cells. Our results disagree on several points with activities of 35S and 34S promoter derivatives reported for tobacco, indicating that absolute and relative promoter activities can differ between plant species.

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References

  1. Benfey PN, Chua N-H: Regulated genes in transgenic plants. Science 244: 174–181 (1989).

    Google Scholar 

  2. Bilang R, Iida S, Peterhans A, Potrykus I, Paszkowski J: The 3′-terminal region of the hygromycin-B-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum. Gene 100: 247–250 (1991).

    Google Scholar 

  3. Jefferson RA: Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).

    Google Scholar 

  4. Fang R-X, Nagy F, Sivasubramaniam S, Chua N-H: Multiple cis regulatory elements for maximal expression of the cauli-flower mosaic virus 35S promoter in transgenic plants. Plant Cell 1: 141–150 (1989).

    Google Scholar 

  5. Holtorf S, Apel K, Bohlmann H: Comparison of different constitutive and inducible promoters for the overexpression of transgenes in Arabidopsis thaliana. Plant Mol Biol 29: 637–646 (1995).

    Google Scholar 

  6. Kay R, Chan A, Daly M, McPherson J: Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236: 1299–1302 (1987).

    Google Scholar 

  7. Klein TM, Wolf ED, Wu R, Sanford JC: High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70–73 (1987).

    Google Scholar 

  8. Lam E, Benfey PN, Gilmartin P, Fang R-X, Chua N-H: Sitespecific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci USA 86: 7890–7894 (1989).

    Google Scholar 

  9. Lam E, Chua N-H: GT-1 binding site confers light responsive expression in transgenic tobacco. Science 248: 471–474 (1990).

    Google Scholar 

  10. Lambé P, Dinant M, Matagne RF: Differential long-term expression and methylation of the hygromycin phosphotransferase (hph) and α-glucuronidase (GUS) genes in transgenic pearl millet (Pennisetum glaucum) callus. Plant Sci 108: 51–62 (1995).

    Google Scholar 

  11. Meijer AH, Verpoorte R, Hoge JHC: Regulation of enzymes and genes involved in terpenoid indole alkaloid biosynthesis in Catharanthus roseus. J Plant Res (special issue) 3: 145–164 (1993).

    Google Scholar 

  12. Mitsuhara I, Ugaki M, Hirochika H, Ohshima M, Murakami T, Gotoh Y, Katayose Y, Nakamura S, Honkura R, Nishimiya S, Ueno K, Mochizuki A, Tanimoto H, Tsugawa H, Otsuki Y, Ohashi Y: Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous andmonocotyledonous plants. Plant Cell Physiol 37: 49–59 (1996).

    Google Scholar 

  13. Neuman JR, Morenay CA, Russian KO: A novel rapid assay for chloramphenicol acetyltransferase gene expression. Biotechniques 5: 444–447 (1987).

    Google Scholar 

  14. Odell JT, Nagy F, Chua N-H: Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313: 810–812 (1985).

    Google Scholar 

  15. Pasquali G, Ouwerkerk PBF, Memelink J: Versatile transformation vectors to assay the promoter activity of DNA elements in plants. Gene 149: 373–374 (1994).

    Google Scholar 

  16. Sanger M, Daubert S, Goodman RM: Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter. Plant Mol Biol 14: 433–443 (1990).

    Google Scholar 

  17. Wilmink A, van de Ven BCE, Dons JJM: Activity of constitutive promoters in various species from the Liliaceae. Plant Mol Biol 28: 949–955 (1995).

    Google Scholar 

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Authors and Affiliations

  1. Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, the Netherlands

    Leslie van der Fits & Johan Memelink

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  1. Leslie van der Fits
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van der Fits, L., Memelink, J. Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment. Plant Mol Biol 33, 943–946 (1997). https://doi.org/10.1023/A:1005763605355

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