Abstract
The promoter of a gene encoding a ribosome-associated protein of 40 kDa from Arabidopsis thaliana (A-p40) was sequenced and the expression of the gene studied. A-p40 was expressed in the same organs and with the same variations as the eukaryotic elongation factor 1α (eEF1A), another gene coding for a protein involved in translation Arabidopsis plants transformed with a β-glucuronidase (GUS) gene driven by the A-p40 promoter confirm that A-p40 is expressed in actively dividing and growing cells. eEF1A promoter-GUS fusions have the same pattern of expression. Comparison of cis-acting elements from A-p40 and eEF1A revealed some common elements. A-p40 promoter deletions and transient gene expression in transfected Arabidopsis protopasts allowed the identification of trap40, a cis-acting element regulating gene expression. Gel retardation experiments indicate that eEF1A and A-p40 are regulated by different cis-acting elements. The role of such elements is discussed.
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Scheer, I., Ludevid, M.D., Regad, F. et al. Expression of a gene encoding a ribosomal p40 protein and identification of an active promoter site. Plant Mol Biol 35, 905–913 (1997). https://doi.org/10.1023/A:1005956601270
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DOI: https://doi.org/10.1023/A:1005956601270