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Sequential and structural homology between intracellular pathogenesis-related proteins and a group of latex proteins

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Abstract

The intracellular pathogenesis-related proteins have been identified in a broad range of flowering plants. Some display quite different patterns of expression, in many cases unrelated to the pathogenic response. Nevertheless, these proteins are all very similar and in most cases share more than 35% sequence identity. In this report we investigate the significance of a rather weak similarity between the intracellular pathogenesis-related (IPR or PR-10) proteins and a group of proteins identified in the latex of opium poppy and in Arabidopsis, among others. A sequence analysis held together with the recently published three-dimensional structure of Bet v 1, an IPR protein from birch pollen, strongly suggests sequential and structural homology between the two protein families.

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References

  1. Bufe A, Spangfort MD, Kählert H, Schlaak M, Becker W-M: The major birch pollen allergen, Bet v 1, shows ribonuclease activity. Planta 199: 413–415 (1996).

    Google Scholar 

  2. Crowell DN, John ME, Russell D, Amasino RM: Characterization of a stress-induced, developmentally regulated gene family from soybean. Plant Mol Biol 18: 459–466 (1992).

    Google Scholar 

  3. Gajhede M, Osmark P, Poulsen FM, Ipsen H, Larsen JN, van Neerven RJJ, Schou C, Lowenstein H, Spangfort MD: X-Ray and NMR structure of Bet v 1, the origin of birch pollen allergy. Nature Struct Biol 3: 1040–1045 (1996).

    Google Scholar 

  4. Matton DP, Brisson N: Cloning, expression, and sequence conservation of pathogenesis-related gene transcripts of potato. Mol Plant-Microbe Interact 2: 325–331 (1989).

    Google Scholar 

  5. Moiseyev GP, Fedoreyeva LI, Zhuravlev YN, Yastnetskaya E, Jekel PA, Beintema JJ: Primary structures of two ribonucleases from ginseng calluses; new members of the PR-10 family of intracellular pathogenesis-related plant proteins. FEBS Lett 407: 207–210 (1997).

    Google Scholar 

  6. Nessler CL, Burnett RJ: Organization of the major latex protein gene family in opium poppy. Plant Mol Biol 20: 749–752 (1992).

    Google Scholar 

  7. Nessler CL, Kurz WGW, Pelcher LE: Isolation and analysis of the major latex protein genes of opium poppy. Plant Mol Biol 15: 951–953 (1990).

    Google Scholar 

  8. Pozueta-Romero J, Klein M, Houlné G, Schantz M-L, Meyer B, Schantz R: Characterization of a family of genes encoding a fruit-specific wound-stimulated protein of bell pepper (Capsicum annuum): identification of a new family of transposable elements. Plant Mol Biol 28: 1011–1025 (1995).

    Google Scholar 

  9. Rost B, Sander C: Prediction of protein secondary structure at better than 70-percent accuracy. J Mol Biol 232: 584–599 (1993).

    Google Scholar 

  10. Rost B, Sander C: Combining evolutionary information and neural networks to predict protein secondary structure. Proteins 19: 55–72 (1994).

    Google Scholar 

  11. Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22: 4673–4680 (1994).

    Google Scholar 

  12. van Loon LC, Pierpoint WS, Boller T, Conejero V: Recommendations for naming plant pathogenesis-related proteins. Plant Mol Biol Rep 12: 245–264 (1994).

    Google Scholar 

  13. Warner SAJ, Scott R, Draper J: Characterisation of a woundinduced transcript from the monocot asparagus that shares similarity with a class of intracellular pathogenesis-related (PR) proteins. Plant Mol Biol 19: 555–561 (1992).

    Google Scholar 

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Osmark, P., Boyle, B. & Brisson, N. Sequential and structural homology between intracellular pathogenesis-related proteins and a group of latex proteins. Plant Mol Biol 38, 1243–1246 (1998). https://doi.org/10.1023/A:1006060224012

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  • DOI: https://doi.org/10.1023/A:1006060224012

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