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Isolation and characterization of mRNAs differentially expressed during ripening of wild strawberry (Fragaria vesca L.) fruits

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Abstract

Wild strawberry (Fragaria vesca L.) is an attactive model system for studying ripening in non-climacteric fruit, because of its small diploid genome, its short reproductive cycle, and its capacity for transformation. We have isolated eight ripening-induced cDNAs from this species after differential screening of a cDNA library. The predicted polypeptides of seven of the clones exhibit similarity to database protein sequences, including acyl carrier protein, caffeoyl- CoA 3-O-methyltransferase, sesquiterpene cyclase, major latex protein, cystathionine γ-synthase, dehydrin and an auxin- induced gene. A ninth cDNA clone that was constitutively expressed is predicted to encode a metallothionein-like protein. None of these proteins appear to be directly related to events generally associated with ripening such as cell wall metabolism or the accumulation of sugars and pigments, rather, their putative functions are indicative of the wide range of processes upregulated during fruit ripening.

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Author notes

  1. Young-Woo Nam

    Present address: Crop Biotechnology Center, and the Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843, USA

Authors and Affiliations

  1. INRA, Station de Technologie des Produits Végétaux, B.P. 91, 84914, Avignon Cédex 9, France

    Line Tichit, Monique Leperlier, Blondine Cuerq & Isabelle Marty

  2. ENSAT, U.A. INRA-ENSAT, Ethylène et Maturation des Fruits, Avenue de l'Agrobiopole, B.P. 107, 31326, Castanet-Tolosan Cédex, France

    Jean-Marc Lelièvre

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Nam, YW., Tichit, L., Leperlier, M. et al. Isolation and characterization of mRNAs differentially expressed during ripening of wild strawberry (Fragaria vesca L.) fruits. Plant Mol Biol 39, 629–636 (1999). https://doi.org/10.1023/A:1006179928312

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