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Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought and cold stresses

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Abstract

A cDNA clone encoding a dehydrin gene was isolated from a cDNA library prepared from white spruce (Picea glauca) needle mRNAs. The cDNA, designated PgDhn1, is 1159 nucleotides long and has an open reading frame of 735 bp with a deduced amino acid sequence of 245 residues. The PgDhn1 amino acid sequence is highly hydrophilic and possesses four conserved repeats of the characterized lysine-rich K-segment (EKKGIMDKIKEKLPG), and an 8-serine residue stretch prior to the first lysine-rich repeat that is common to many dehydrins. The DEYGNP conserved motif is, however, absent in the PgDhn1 sequence. In unstressed plants, the highest level of transcripts was detected in stem tissue and not fully expanded vegetative buds. PgDhn1 expression was also clearly detected in reproductive buds, at various stages of development. The mRNAs corresponding to PgDhn1 cDNA were induced upon wounding and by jasmonic acid (JA) and methyl jasmonate (MeJa) treatments. Upon drought stress, increased transcript accumulation was observed in needle tissue reaching a maximum level 48 h after treatment. Treatments of seedlings with abscisic acid or ethephon also resulted in high levels of transcript accumulation in needle tissue. Finally, cold induction of PgDhn1 transcripts was also detected as early as 8 h after treatment.

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Authors and Affiliations

  1. Centre de recherche en biologie forestière, Université Laval, Pavillon C.-E.-Marchand, Sainte-Foy, Québec, Canada, G1K 7P4

    Sylvie Richard & Armand Séguin

  2. Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, 1055 du P.E.P.S., P.O. Box 3800, Sainte-Foy, Québec, Canada, G1V 4C7

    Marie-Josée Morency & Armand Séguin

  3. Laboratoire de biologie cellulaire, Institut National de la Recherche Agronomique, route de Saint-Cyr, 78026, Versailles Cedex, France

    Christine Drevet & Lise Jouanin

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  1. Sylvie Richard
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  2. Marie-Josée Morency
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  3. Christine Drevet
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  4. Lise Jouanin
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  5. Armand Séguin
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Richard, S., Morency, MJ., Drevet, C. et al. Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought and cold stresses. Plant Mol Biol 43, 1–10 (2000). https://doi.org/10.1023/A:1006453811911

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