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Isolation of ergosterol peroxide and its reversion to ergosterol in the pathogenic fungus Sporothrix schenckii

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Abstract

Ergosterol peroxide, a presumed product of the H_2O_2-dependent enzymatic oxidation of ergosterol, has been isolated from yeast forms of the pathogenic fungus Sporothrix schenckii. The substance, which may have a role in fungal virulence, has been characterized mainly using spectroscopic methods (1H and 13C nuclear magnetic resonance and high resolution mass spectra). The purified compound showed a molecular formula of C_28H_44O_3, displaying characteristic features of epidioxy sterols and was reverted to ergosterol when submitted to S. schenckii enzymatic extract.

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Authors and Affiliations

  1. Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niteríi, RJ

    Diana Bridon da Graça Sgarbi

  2. Núcleo de Pesquisas em Produtos Naturais, Universidade Federal do Rio de Janeiro, RJ

    Antonio Jorge Ribeiro da Silva

  3. Departamento de Análises Clinicas, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Paulista Júlio Mesquita Filho, Araraquara, SP

    Iracilda Zeppone Carlos

  4. Departamento de Parasitologia, Microbiologia e Imunologia, Escola de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP e

    Célio Lopes Silva

  5. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, RJ, Brasil

    Jayme Angluster & Celuta Sales Alviano

Authors
  1. Diana Bridon da Graça Sgarbi
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  2. Antonio Jorge Ribeiro da Silva
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  3. Iracilda Zeppone Carlos
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  4. Célio Lopes Silva
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  5. Jayme Angluster
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  6. Celuta Sales Alviano
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da Graça Sgarbi, D.B., da Silva, A.J.R., Carlos, I.Z. et al. Isolation of ergosterol peroxide and its reversion to ergosterol in the pathogenic fungus Sporothrix schenckii. Mycopathologia 139, 9–14 (1997). https://doi.org/10.1023/A:1006803832164

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  • DOI: https://doi.org/10.1023/A:1006803832164

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