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Envelope and Long Terminal Repeat Sequences of an Infectious Murine Leukemia Virus from a Human SCLC Cell Line: Implications for Gene Transfer

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Abstract

Development of methods for gene transfer into specific cell types or tissues is important for experimental research as well as clinical therapeutical approaches. We report here the cloning and characterization of the envelope (env) gene and the U3 region of a retrovirus from an infected human Small Cell Lung Cancer (SCLC) cell line. The replication of this murine retrovirus is also fully supported by other lung cancer cell lines of different histological origin. We present evidence that a long terminal repeat (LTR)-β-galactosidase (β-Gal) reporter construct performed as well as an analogous cytomegalovirus (CMV) promoter β-Gal construct in the human lung epithelial cell line A549 and in the human larynx carcinoma cell line HEp2.

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Authors and Affiliations

  1. Ruhr-Universita¨t Bochum, Medizinische Fakulta¨t, Institut fu¨r Hygiene und Mikrobiologie, Abteilung fu¨r Med. Mikrobiol. und Virologie, Universita¨tsstraße 150, D-44780 Bochum, Geba¨ude MA 6/130, Germany

    Marianne Antoine & Paul Kiefer

  2. Philipps Universita¨t Marburg, Zentrum fu¨r Innere Medizin, Abteilung fu¨r Ha¨matologie/Onkologie, Baldingerstraße, D-3550, Marburg, Germany

    Barbara Wegmann

Authors
  1. Marianne Antoine
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  2. Barbara Wegmann
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  3. Paul Kiefer
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Antoine, M., Wegmann, B. & Kiefer, P. Envelope and Long Terminal Repeat Sequences of an Infectious Murine Leukemia Virus from a Human SCLC Cell Line: Implications for Gene Transfer. Virus Genes 17, 157–168 (1998). https://doi.org/10.1023/A:1008020808314

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