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Role of HSP70i in regulation of biomaterial-induced activation of human monocytes-derived macrophages in culture

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Abstract

The functioning of an implant depends on the material properties and the wound-healing process. The latter is led by an inflammatory reaction guided mainly by monocyte/macrophage activity. This in vitro study investigated human monocytes/macrophages in culture from 2 h to 10 days on silicone, polyurethane, teflon and TCPS. Analysis of cytokine release by ELISA showed that maturing macrophages have different capacities to produce cytokines TNFα, IL10, IL8 and GM-CSF. The long culture-mature macrophages on all polymers produced comparable low levels of TNFα, IL10 and IL8. Monocytes/macrophages on polyurethane and teflon, and those on silicone only in long culture-time produced high GM-CSF amounts, where as those on TCPS exhibited low levels of GM-CSF. FACS analysis revealed that HSP70i was highly inducible after short time culture yet this high level was maintained in long culture-mature macrophages on TCPS only, whereas on other polymers the mature macrophages showed a high reduction in HSP70i level, which demonstrated a high stress-response by cells on TCPS. Accordingly, CLSM-analysis revealed low nuclear NF-κB in cells on TCPS and high nuclear NF-κB in mature macrophages on silicone and polyurethane, showing a high cellular activation on the latter two polymers. This corresponded also to the high mitochondrial activity by XTT metabolism displayed by the mature macrophages on polyurethane ≥ silicone > teflon > TCPS. These data show a correlation of (1) cytokines (TNFα, GM-CSF) and HSP70i, (2) NF-κB and HSP70i by monocytes/macrophages after contact with polymers. Thus, HSP70i might be a useful molecular candidate for exploring biomaterial-induced inflammatory reaction.

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Authors and Affiliations

  1. IZKF“BIOMAT”, The Technical University of Aachen, Pauwels Str. 30, D-52057, Aachen, Germany

    R. S. Bhardwaj

  2. DWI, The Technical University of Aachen, Pauwels Str. 30, D-52057, Aachen, Germany

    M. Eblenkamp & T. Berndt

  3. Institute of Pathology, The Technical University of Aachen, Pauwels Str. 30, D-52057, Aachen, Germany

    L. Tietze & B. Klosterhalfen

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Bhardwaj, R.S., Eblenkamp, M., Berndt, T. et al. Role of HSP70i in regulation of biomaterial-induced activation of human monocytes-derived macrophages in culture. Journal of Materials Science: Materials in Medicine 12, 97–106 (2001). https://doi.org/10.1023/A:1008974524580

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