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Interstitial colocalization of two cervid satellite DNAs involved in the genesis of the Indian muntjac karyotype

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Abstract

A number of repetitive DNA clones were generated from PCR amplifications of Indian muntjac genomic DNA using primer sequences derived from a white tailed deer satellite II DNA sequence. One clone (Mmv-0.7) was characterized and shown to be a cervid satellite II DNA clone. Multiple colored FISH studies with cervid satellite I (C5) and this satellite II clone (Mmv-0.7) to Chinese muntjac metaphase chromosomes localized both satellite DNAs at the pericentromeric regions of all chromosomes except for chromosome 3 and the Y chromosome, whereas chromosome 3 exhibited pericentromeric satellite II DNA only. Where distinguishable, the pericentromeric satellite II signals appeared terminally oriented with respect to satellite I. Six pairs of Chinese muntjac autosomes had interstitial satellite I sites with four of these autosomal pairs (chromosomes 1, 2 and two other smaller autosomal pairs) also exhibiting interstitial satellite II signals. An interstitial site on the X chromosome was found to have satellite II signals. For the Indian muntjac chromosomes, FISH studies revealed a pericentromeric hybridization for satellites I and II as well as 27 distinct interstitial hybridization sites, each having at least one of the satellite DNAs. These data were used to more precisely define the chromosome fusion-associated breakpoints that presumably led to the formation of the present-day Indian muntjac karyotype. It further hints at the possibility that the Indian muntjac karyotype may have evolved directly from a 2n=70 ancestral karyotype rather than from an intermediate 2n=46 Chinese muntjac-like karyotype.

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Authors and Affiliations

  1. Department of Life Sciences, Chung Shan Medical and Dental College, No. 110, Sec. 1, Chien Kuo N. Rd., Taichung, Taiwan, 40203

    Yueh-Chun Li & Shuan-Yow Li

  2. Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA

    Charles Lee & Chyi-Chyang Lin

  3. Department of Pathology, University of Cambridge, Cambridge, England, CB2 1QP

    Despina Sanoudou

  4. Department of Life Sciences, National Tsing Hwa University, Hsinchu, Taiwan

    Tzong-Hsiung Hsu

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  1. Yueh-Chun Li
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  2. Charles Lee
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  3. Despina Sanoudou
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  4. Tzong-Hsiung Hsu
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  5. Shuan-Yow Li
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Li, YC., Lee, C., Sanoudou, D. et al. Interstitial colocalization of two cervid satellite DNAs involved in the genesis of the Indian muntjac karyotype. Chromosome Res 8, 363–373 (2000). https://doi.org/10.1023/A:1009203518144

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