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Anti-Galectin-3 IgG Autoantibodies in Patients with Crohn's Disease Characterized by Means of Phage Display Peptide Libraries

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Abstract

Galectin-3, a member of β-galactoside-binding lectins, is expressed and secreted by a variety of cell types including human intestinal epithelial cells. The presence of anti–galectin-3 antibody in the sera of patients was analyzed by immunoblotting using recombinant human galectin-3. A substantially higher percentage of sera from Crohn's disease patients contained anti-galectin-3 IgG autoantibodies than from patients with ulcerative colitis, primary biliary cirrhosis, or autoimmune hepatitis and of apparently healthy control volunteers. In Crohn's disease patients the titer of autoantibodies was high and interestingly correlated negatively with disease activity. To characterize and generate artificial epitopes (mimotopes), the anti-galectin-3 monoclonal antibodies A3A12 and B2C10 were used for biopannings of phage display nonapeptide libraries. These mimotopes interfered with the binding of autoantibodies to recombinant and native intestinal epithelial galectin-3. Our data may suggest that galectin-3 mimotopes could be used for the induction of IgG with desired specificity to regulate immune responses in Crohn's disease patients.

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Authors and Affiliations

  1. Department of Pathophysiology, University of Vienna, Vienna, Austria

    Erika Jensen-Jarolim, Christian Neumann, Regina Gscheidlinger & George Boltz-Nitulescu

  2. Department of Clinical Pathology, University of Vienna, Vienna, Austria

    Georg Oberhuber

  3. Clinic of Oto-Rhino-Laryngology, University of Vienna, Vienna, Austria

    Csilla Neuchrist

  4. Clinic of Gastroenterology and Hepatology, University of Vienna, Vienna, Austria

    Walter Reinisch & Edward Penner

  5. Division of Allergy, La Jolla Institute for Allergy and Immunology, San Diego, CA

    Riaz I. Zuberi & Fu-Tong Liu

Authors
  1. Erika Jensen-Jarolim
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  2. Christian Neumann
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  3. Georg Oberhuber
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  4. Regina Gscheidlinger
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  5. Csilla Neuchrist
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  6. Walter Reinisch
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  7. Riaz I. Zuberi
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  8. Edward Penner
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  9. Fu-Tong Liu
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  10. George Boltz-Nitulescu
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Jensen-Jarolim, E., Neumann, C., Oberhuber, G. et al. Anti-Galectin-3 IgG Autoantibodies in Patients with Crohn's Disease Characterized by Means of Phage Display Peptide Libraries. J Clin Immunol 21, 348–356 (2001). https://doi.org/10.1023/A:1012240719801

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